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Development and Evaluation of a Reverse-Entry Ion Source Orbitrap Mass Spectrometer

Authors :
David H. Russell
Jacob W. McCabe
Michael L. Poltash
John W. Patrick
Arthur Laganowsky
Source :
Journal of the American Society for Mass Spectrometry. 30:192-198
Publication Year :
2018
Publisher :
American Chemical Society (ACS), 2018.

Abstract

As a step towards development of a high-resolution ion mobility mass spectrometer using the orbitrap mass analyzer platform, we describe herein a novel reverse-entry ion source (REIS) coupled to the higher-energy C-trap dissociation (HCD) cell of an orbitrap mass spectrometer with extended mass range. Development of the REIS is a first step in the development of a drift tube ion mobility-orbitrap MS. The REIS approach retains the functionality of the commercial instrument ion source which permits the uninterrupted use of the instrument during development as well as performance comparisons between the two ion sources. Ubiquitin (8.5 kDa) and lipid binding to the ammonia transport channel (AmtB, 126 kDa) protein complex were used as model soluble and membrane proteins, respectively, to evaluate the performance of the REIS instrument. Mass resolution obtained with the REIS is comparable to that obtained using the commercial ion source. The charge state distributions for ubiquitin and AmtB obtained on the REIS are in agreement with previous studies which suggests that the REIS-orbitrap EMR retains native structure in the gas phase.

Details

ISSN :
10440305
Volume :
30
Database :
OpenAIRE
Journal :
Journal of the American Society for Mass Spectrometry
Accession number :
edsair.doi.dedup.....6d8878e20d5ed1aaea9ee33124171fc2
Full Text :
https://doi.org/10.1007/s13361-018-1976-0