Requirement of myomaker-mediated stem cell fusion for skeletal muscle hypertrophy

Fusion of skeletal muscle stem/progenitor cells is required for proper development and regeneration, however the significance of this process during adult muscle hypertrophy has not been explored. In response to muscle overload after synergist ablation in mice, we show that myomaker, a muscle specific membrane protein essential for myoblast fusion, is activated mainly in muscle progenitors and not myofibers. We rendered muscle progenitors fusion-incompetent through genetic deletion of myomaker in muscle stem cells and observed a complete reduction of overload-induced hypertrophy. This blunted hypertrophic response was associated with a reduction in Akt and p70s6k signaling and protein synthesis, suggesting a link between myonuclear accretion and activation of pro-hypertrophic pathways. Furthermore, fusion-incompetent muscle exhibited increased fibrosis after muscle overload, indicating a protective role for normal stem cell activity in reducing myofiber strain associated with hypertrophy. These findings reveal an essential contribution of myomaker-mediated stem cell fusion during physiological adult muscle hypertrophy. DOI: http://dx.doi.org/10.7554/eLife.20007.001
eLife digest Skeletal muscle has a remarkable capacity to adapt to a variety of stimuli, including an ability to become larger and stronger through exercise. In embryos, new muscles develop from muscle stem cells, which either replicate themselves or “differentiate” into mature muscle cells. Adult muscles also contain stem cells, which are normally dormant, but activate when the muscle is damaged. The stem cells subsequently differentiate and fuse with one another or to existing muscle fibers to restore the muscle. What is not fully understood is whether this fusion process also helps undamaged adult muscles to increase in size (for example, in response to exercise). Fusion proteins such as myomaker – which specifically acts in muscles – help the stem cells to fuse. To investigate myomaker’s role in adult muscle growth, Goh and Millay deleted the gene that produces it from the muscle stem cells of mice. The mice then experienced two weeks of increased muscle activity, after which their muscle growth was compared with that of normal mice that had been subjected to the same activity routine. Goh and Millay discovered that myomaker is important in muscle stem cells, and not in muscle fibers, for adult muscle growth. After two weeks of increased muscle activity, substantial levels of muscle stem cell fusion had occurred in normal mice, and their muscles had grown significantly. However, the muscles of mice that lacked myomaker in their muscle stem cells did not increase in size. Additional experiments showed that normal muscle stem cell fusion activates signaling pathways that create new proteins and drive muscle growth. Furthermore, scarring occurred in muscles that lacked myomaker, suggesting that stem cell fusion also protects muscle fibers from damage during increased activity. Overall, the findings presented by Goh and Millay reveal that the fusion of muscle stem cells is an important event for adult muscle growth. Further studies are now needed to determine the relevance of muscle stem cell fusion during the normal aging process, and to uncover the relationship between fusion and the activation of pro-growth signaling pathways. DOI: http://dx.doi.org/10.7554/eLife.20007.002