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Comparison of four commercially available avidity tests for Toxoplasma gondii-specific IgG antibodies
- Source :
- Clinical and Vaccine Immunology, Clinical and Vaccine Immunology, American Society for Microbiology, 2013, 20 (2), pp.197-204. ⟨10.1128/cvi.00356-12⟩
- Publication Year :
- 2013
- Publisher :
- HAL CCSD, 2013.
-
Abstract
- Toxoplasma infection in pregnant women may cause congenital toxoplasmosis. Diagnosis of infection is based on serological tests aimed at detecting IgM and IgG antibodies against Toxoplasma gondii . However, IgM antibodies are not an accurate marker for discriminating between acute and latent infection. Detection of residual or persistent IgM may occur months or even years after primary infection, while the IgG avidity test is a rapid means of identifying latent infections in pregnant women who exhibit both IgG and IgM anti- Toxoplasma antibodies on initial testing during pregnancy. In this study, we assessed and compared the performances of four commercially available Toxoplasma IgG avidity tests in immunocompetent and immunocompromised patients with acute and latent toxoplasmosis. The positive predictive value of high avidity to confirm latent toxoplasmosis was 100% for all the assays, indicating that high avidity is a hallmark of latent infection. However, the negative predictive value of high avidity ranged from 99.2% (bioMérieux) to 95.3% (Abbott), indicating that acute toxoplasmosis could not be reliably diagnosed based on low IgG avidity alone. Thus, the avidity test provides a rapid means for identifying latent Toxoplasma infection in immunocompetent pregnant women presenting both IgG and IgM anti- Toxoplasma antibodies on initial testing. In terms of cost-effectiveness, avidity testing is a powerful tool that optimizes screening and follow-up of pregnant women while minimizing the costs of screening by avoiding subsequent costly maternal and fetal investigation and unnecessary treatment. The cheapest assay, Vidas Toxo IgG Avidity, also had the best performance for the diagnosis of latent toxoplasmosis.
- Subjects :
- diagnosis
multicenter evaluation
Clinical Biochemistry
MESH: Reagent Kits, Diagnostic
Antibody Affinity
Antibodies, Protozoan
Immunoglobulin G
Serology
0302 clinical medicine
MESH: Pregnancy
MESH: Antibody Affinity
Pregnancy
Immunology and Allergy
toxo igg
MESH: Immunoglobulin G
0303 health sciences
immunoglobulin-g igg
biology
MESH: Toxoplasma
MESH: Immunoglobulin M
3. Good health
immunocompetent pregnant-women
MESH: Toxoplasmosis
Female
Antibody
Toxoplasma
toxoplasmosis
Microbiology (medical)
030231 tropical medicine
Immunology
chemical and pharmacologic phenomena
system
03 medical and health sciences
Diagnostic Laboratory Immunology
parasitic diseases
medicine
Humans
MESH: Antibodies, Protozoan
Avidity
MESH: Humans
030306 microbiology
business.industry
congenital
Toxoplasma gondii
assay
medicine.disease
biology.organism_classification
Virology
Toxoplasmosis
Immunoglobulin M
biology.protein
[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie
Reagent Kits, Diagnostic
business
MESH: Female
Subjects
Details
- Language :
- English
- ISSN :
- 15566811
- Database :
- OpenAIRE
- Journal :
- Clinical and Vaccine Immunology, Clinical and Vaccine Immunology, American Society for Microbiology, 2013, 20 (2), pp.197-204. ⟨10.1128/cvi.00356-12⟩
- Accession number :
- edsair.doi.dedup.....6d5da8ae457ad8b065e3374e5f48e786
- Full Text :
- https://doi.org/10.1128/cvi.00356-12⟩