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Heterogeneity of penicillin-non-susceptible group B streptococci isolated from a single patient in Germany
- Source :
- Journal of Antimicrobial Chemotherapy
- Publication Year :
- 2019
- Publisher :
- Oxford University Press (OUP), 2019.
-
Abstract
- Objectives Streptococcus agalactiae [group B streptococci (GBS)] have been considered uniformly susceptible to penicillin. However, increasing reports from Asia and North America are documenting penicillin-non-susceptible GBS (PRGBS) with mutations in pbp genes. Here we report, to the best of our knowledge, the first two PRGBS isolates recovered in Europe (AC-13238-1 and AC-13238-2), isolated from the same patient. Methods Two different colony morphologies of GBS were noted from a surgical abscess drainage sample. Both were serotyped and antimicrobial susceptibility testing was performed by different methodologies. High-throughput sequencing was done to compare the isolates at the genomic level, to identify their capsular type and ST, to evaluate mutations in the pbp genes and to compare the isolates with the genomes of other PRGBS isolates sharing the same serotype and ST. Results Isolates AC-13238-1 and AC-13238-2 presented MICs above the EUCAST and CLSI breakpoints for penicillin susceptibility. Both shared the capsular type Ia operon and ST23. Genomic analysis uncovered differences between the two isolates in seven genes, including altered pbp genes. Deduced amino acid sequences revealed critical substitutions in PBP2X in both isolates. Comparison with serotype Ia clonal complex 23 PRGBS from the USA reinforced the similarity between AC-13238-1 and AC-13238-2, and their divergence from the US strains. Conclusions Our results support the in-host evolution of β-lactam-resistant GBS, with two PRGBS variants being isolated from one patient.
- Subjects :
- 0301 basic medicine
Microbiology (medical)
Serotype
Operon
Penicillin Resistance
030106 microbiology
Microbial Sensitivity Tests
Penicillins
Biology
medicine.disease_cause
Genome
Group B
Streptococcus agalactiae
Microbiology
03 medical and health sciences
0302 clinical medicine
Germany
Streptococcal Infections
medicine
Humans
Pharmacology (medical)
030212 general & internal medicine
Gene
Original Research
Pharmacology
Mutation
Anti-Bacterial Agents
Penicillin
Infectious Diseases
medicine.drug
Subjects
Details
- ISSN :
- 14602091 and 03057453
- Volume :
- 75
- Database :
- OpenAIRE
- Journal :
- Journal of Antimicrobial Chemotherapy
- Accession number :
- edsair.doi.dedup.....6d55fa7280bcb4652e19422b558ace3d