Back to Search
Start Over
A candidate gene study of one-carbon metabolism pathway genes and colorectal cancer risk
- Source :
- The British journal of nutrition. 109(6)
- Publication Year :
- 2012
-
Abstract
- The risk of colorectal cancer (CRC) may be influenced by aberrant DNA methylation and altered nucleotide synthesis and repair, possibly caused by impaired dietary folate intake as well as by polymorphic variants in one-carbon metabolism genes. A case–control study using seventy-one CRC patients and eighty unrelated healthy controls was carried out to assess the genetic association of fifteen SNP and one insertion in nine genes belonging to the folate pathway. Polymorphism selection was based on literature data, and included those which have a known or suspected functional impact on cancer and missense polymorphisms that are most likely to alter protein function. Genotyping was performed by real-time PCR and PCR followed by restriction analysis. The likelihood ratio statistic indicated that most of the polymorphisms were not associated with the risk of CRC. However, an increased risk of CRC was observed for two variant alleles of SNP mapping on the transcobalamin 2 gene (TCN2): C776G (rs1801198) and c.1026-394T>G (rs7286680). Considering the crucial biological function played by one-carbon metabolism genes, further investigations with larger cohorts of CRC patients are needed in order to confirm our preliminary results. These preliminary results indicate that TCN2 polymorphisms can be a susceptibility factor for CRC.
- Subjects :
- Male
Candidate gene
Genotype
Colorectal cancer
METHYLENETETRAHYDROFOLATE REDUCTASE, FOLATE METABOLISM, COLON-CANCER, MTHFR C677T, POLYMORPHISMS, TRANSCOBALAMIN, ADENOMA, METAANALYSIS, ASSOCIATION
Medicine (miscellaneous)
Biology
Polymerase Chain Reaction
Polymorphism, Single Nucleotide
Folic Acid
Methionine
Gene Frequency
medicine
SNP
Humans
Genetic Predisposition to Disease
Gene
Genotyping
Genetic Association Studies
Genetic association
Aged
One-Carbon Group Transferases
Genetics
Transcobalamins
Nutrition and Dietetics
Nucleotides
Cancer
medicine.disease
Carbon
Diet
Mutagenesis, Insertional
Vitamin B 12
Methylenetetrahydrofolate reductase
Case-Control Studies
biology.protein
Female
Colorectal Neoplasms
Subjects
Details
- ISSN :
- 14752662
- Volume :
- 109
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- The British journal of nutrition
- Accession number :
- edsair.doi.dedup.....6d54c8b3bd5de5bab3b2d268cdbebda8