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Precision Cancer Medicine in the Acoustic Dispensing Era: Ex Vivo Primary Cell Drug Sensitivity Testing
- Source :
- SLAS Technology. 21:27-36
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- Cancer therapy is increasingly becoming individualized, but there are also big gaps between the molecular knowledge of individual cancers we can generate today and what can be applied in the clinic. In an attempt to bridge this knowledge gap between cancer genetic and molecular profiling and clinically useful information, an individualized systems medicine program has been established at the Institute for Molecular Medicine Finland (FIMM), University of Helsinki, and the Helsinki University Hospital. Central to this program is drug sensitivity and resistance testing (DSRT), in which responses of primary cancer cells to a comprehensive clinical oncology and signal transduction drug collection are monitored. The drug sensitivity information is used with molecular profiling to establish hypotheses on individual cancer-selective targeting drug combinations and their predictive biomarkers, which can be explored in the clinic. Here, we describe how acoustic droplet ejection is enabling DSRT in our cancer individualized systems medicine program to (1) generate consistent but configurable assay-ready plates and determine how this affects data quality, (2) flexibly prepare drug combination testing plates, (3) dispense reagents and cells to the assay plates, and (4) perform ultra-miniaturized follow-up assays on the cells from DSRT plates.
- Subjects :
- 0301 basic medicine
Drug
medicine.medical_specialty
Cell Survival
media_common.quotation_subject
Cytological Techniques
Compound management
Antineoplastic Agents
Bioinformatics
Hospitals, University
03 medical and health sciences
0302 clinical medicine
Cancer Medicine
Neoplasms
Humans
Medicine
Medical physics
Precision Medicine
Acoustic droplet ejection
Cells, Cultured
Finland
media_common
business.industry
Acoustics
Precision medicine
Molecular medicine
3. Good health
Computer Science Applications
Systems medicine
Medical Laboratory Technology
030104 developmental biology
Drug Resistance, Neoplasm
030220 oncology & carcinogenesis
Drug Screening Assays, Antitumor
business
Ex vivo
Subjects
Details
- ISSN :
- 24726303
- Volume :
- 21
- Database :
- OpenAIRE
- Journal :
- SLAS Technology
- Accession number :
- edsair.doi.dedup.....6d4c5e67b0de8a49d021aab894c18a22