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Identification of microRNA in the protist Trichomonas vaginalis
- Source :
- Genomics. 93(5):487-493
- Publication Year :
- 2009
- Publisher :
- Elsevier BV, 2009.
-
Abstract
- MicroRNAs (miRNAs) are a class of small noncoding RNAs that have important regulatory roles in multicellular organisms. However, miRNA has never been identified experimentally in protist. Direct cloning of 438 expressed miRNA tags by microRNA serial analysis of gene expression from the parasitic protist Trichomonas vaginalis identified nine candidate miRNAs. Bioinformatics analysis of the corresponding genomic region revealed that these miRNA candidates contain a classical stem–loop–stem structure of pre-microRNAs. Analysis of the 20 nt long mature tva-miR-001 showed that it is an intergenic miRNA located at the scaffold DS113596. Tva-miR-001 was differentially expressed in the trophozoite, pseudocyst and amoeboid stages. Based on the experimental results of the present study, we provided solid evidence that protist possesses a miRNA regulating network comparable with multicellular organisms for the first time.
- Subjects :
- Regulation of gene expression
Base Sequence
Protist
Molecular Sequence Data
Chromosome Mapping
MicroRNA
Computational biology
Biology
Genome
Molecular biology
MicroRNAs
Multicellular organism
Gene Expression Regulation
RNA interference
microRNA
Trichomonas vaginalis
Genetics
Animals
Nucleic Acid Conformation
Gene silencing
Amino Acid Sequence
Serial analysis of gene expression
Cloning, Molecular
Genome, Protozoan
Gene
Subjects
Details
- ISSN :
- 08887543
- Volume :
- 93
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Genomics
- Accession number :
- edsair.doi.dedup.....6d4386fbc2bb8749bd34f8d72f7682ba
- Full Text :
- https://doi.org/10.1016/j.ygeno.2009.01.004