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Monitoring peripheral nerve degeneration in ALS by label-free stimulated Raman scattering imaging
- Source :
- Nature Communications, Vol 7, Iss 1, Pp 1-15 (2016), Nature Communications
- Publication Year :
- 2016
- Publisher :
- Nature Portfolio, 2016.
-
Abstract
- The study of amyotrophic lateral sclerosis (ALS) and potential interventions would be facilitated if motor axon degeneration could be more readily visualized. Here we demonstrate that stimulated Raman scattering (SRS) microscopy could be used to sensitively monitor peripheral nerve degeneration in ALS mouse models and ALS autopsy materials. Three-dimensional imaging of pre-symptomatic SOD1 mouse models and data processing by a correlation-based algorithm revealed that significant degeneration of peripheral nerves could be detected coincidentally with the earliest detectable signs of muscle denervation and preceded physiologically measurable motor function decline. We also found that peripheral degeneration was an early event in FUS as well as C9ORF72 repeat expansion models of ALS, and that serial imaging allowed long-term observation of disease progression and drug effects in living animals. Our study demonstrates that SRS imaging is a sensitive and quantitative means of measuring disease progression, greatly facilitating future studies of disease mechanisms and candidate therapeutics.<br />Sensitive and label-free imaging methods to visualize nerve degeneration are currently lacking. Here authors show that stimulated Raman scattering (SRS) microscopy can be used to monitor peripheral nerve degeneration in mouse models of amyotrophic lateral sclerosis (ALS) and in postmortem tissue from ALS patients.
- Subjects :
- Male
0301 basic medicine
Pathology
General Physics and Astronomy
Minocycline
Degeneration (medical)
Electromyography
Spectrum Analysis, Raman
Mice
Superoxide Dismutase-1
0302 clinical medicine
C9orf72
Transgenes
Amyotrophic lateral sclerosis
Myelin Sheath
Motor Neurons
Multidisciplinary
medicine.diagnostic_test
Lipids
Sciatic Nerve
Anti-Bacterial Agents
Peripheral
Disease Progression
Female
Artifacts
Algorithms
medicine.medical_specialty
Science
SOD1
Mice, Transgenic
Article
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
Imaging, Three-Dimensional
medicine
Animals
Humans
Computer Simulation
Peripheral Nerves
Muscle Denervation
business.industry
Amyotrophic Lateral Sclerosis
General Chemistry
medicine.disease
030104 developmental biology
Nerve Degeneration
Trinucleotide repeat expansion
business
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 20411723
- Volume :
- 7
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Nature Communications
- Accession number :
- edsair.doi.dedup.....6d3ceaf283c3d7d8630de828db477271