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The use of regression analysis in determining reference intervals for low hematocrit and thrombocyte count in multiple electrode aggregometry and platelet function analyzer 100 testing of platelet function
- Source :
- Platelets, 28(7), 668-675. Routledge/Taylor & Francis Group
- Publication Year :
- 2017
- Publisher :
- Routledge/Taylor & Francis Group, 2017.
-
Abstract
- Low platelet counts and hematocrit levels hinder whole blood point-of-care testing of platelet function. Thus far, no reference ranges for MEA (multiple electrode aggregometry) and PFA-100 (platelet function analyzer 100) devices exist for low ranges. Through dilution methods of volunteer whole blood, platelet function at low ranges of platelet count and hematocrit levels was assessed on MEA for four agonists and for PFA-100 in two cartridges. Using (multiple) regression analysis, 95% reference intervals were computed for these low ranges. Low platelet counts affected MEA in a positive correlation (all agonists showed r(2) >= 0.75) and PFA-100 in an inverse correlation (closure times were prolonged with lower platelet counts). Lowered hematocrit did not affect MEA testing, except for arachidonic acid activation (ASPI), which showed a weak positive correlation (r(2) = 0.14). Closure time on PFA-100 testing was inversely correlated with hematocrit for both cartridges. Regression analysis revealed different 95% reference intervals in comparison with originally established intervals for both MEA and PFA-100 in low platelet or hematocrit conditions. Multiple regression analysis of ASPI and both tests on the PFA-100 for combined low platelet and hematocrit conditions revealed that only PFA-100 testing should be adjusted for both thrombocytopenia and anemia. 95% reference intervals were calculated using multiple regression analysis. However, coefficients of determination of PFA-100 were poor, and some variance remained unexplained. Thus, in this pilot study using (multiple) regression analysis, we could establish reference intervals of platelet function in anemia and thrombocytopenia conditions on PFA-100 and in thrombocytopenia conditions on MEA.
- Subjects :
- Male
Platelet Aggregation
MULTIPLATE(TM)
Pilot Projects
thrombocytopenia
030204 cardiovascular system & hematology
Hematocrit
DISEASE
VARIABLES
0302 clinical medicine
multiple electrode aggregometry
Reference Values
Platelet
PFA-100(R)
Volunteer
Whole blood
Arachidonic Acid
medicine.diagnostic_test
Regression analysis
Hematology
General Medicine
Middle Aged
anemia
Adenosine Diphosphate
Point-of-Care Testing
Cardiology
Regression Analysis
Female
Platelet function
Collagen
Adult
Blood Platelets
medicine.medical_specialty
Spectrum analyzer
Adolescent
Platelet Function Tests
Anemia
DISORDERS
Point-of-care testing
03 medical and health sciences
Internal medicine
medicine
WHOLE-BLOOD
Humans
Automation, Laboratory
business.industry
AGGREGATION
medicine.disease
platelet function analyzer 100
Case-Control Studies
Immunology
Receptors, Thrombin
business
030215 immunology
Subjects
Details
- Language :
- English
- ISSN :
- 13691635 and 09537104
- Volume :
- 28
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Platelets
- Accession number :
- edsair.doi.dedup.....6d38a9cac441d8e7a5accfd59e9debfc