Comparison of alcalase- and pepsin-treated oilseed protein hydrolysates – Experimental validation of predicted antioxidant, antihypertensive and antidiabetic properties

There is emerging evidence on the importance of food-derived bioactive peptides to promote human health. Compared with animal derived proteins, plant proteins, in particular oilseed proteins, are considered as affordable and sustainable sources of bioactive peptides. Based on our previous bioinformatic analysis, five oilseed proteins (flaxseed, rapeseed, sunflower, sesame and soybean) were enzymatically hydrolysed using alcalase and pepsin (pH 1.3 and pH 2.1). Further, low molecular weight (Mw ​
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Highlights • Bioactive capabilities of protein hydrolysates show overall limited correlation compared to in silico prediction. • Apart from DPP-IV inhibition, low Mw fractions tend to exert more promising biological activities. • In vitro analysis supports oilseed proteins, especially soybean, as good sources of bioactive peptides. • Confirmation of in vitro release of predicted peptides is needed.