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Oligonucleotide-templated lateral flow assays for amplification-free sensing of circulating microRNAs
- Publication Year :
- 2019
- Publisher :
- Royal Society of Chemistry, 2019.
-
Abstract
- Herein we demonstrate the first example of oligonucleotide-templated reaction (OTR) performed on paper, using lateral flow to capture and concentrate specific nucleic acid biomarkers on a test line. Quantitative analysis, using a low-cost benchtop fluorescence reader showed very high specificity down to the single nucleotide level and proved sensitive enough for amplification-free, on-chip, detection of endogenous concentrations of miR-150-5p, a recently identified predictive blood biomarker for preterm birth.
- Subjects :
- Paper
Chemistry, Multidisciplinary
BIOMARKERS
Oligonucleotides
Catalysis
Fluorescence
chemistry.chemical_compound
Materials Chemistry
Humans
NUCLEIC-ACIDS
Circulating MicroRNA
Oligonucleotide Array Sequence Analysis
CELL-FREE MICRORNAS
Science & Technology
Oligonucleotide
Chemistry
Organic Chemistry
Metals and Alloys
RNA
General Chemistry
DNA
Surfaces, Coatings and Films
Electronic, Optical and Magnetic Materials
Biochemistry
Physical Sciences
Ceramics and Composites
Nucleic acid
Biomarker (medicine)
03 Chemical Sciences
Quantitative analysis (chemistry)
PNA
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....6d28f10c01d1f3cb8d371b556d961a7d