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Human T Cell Leukemia Virus Type I-Infected Patients with Hashimoto’s Thyroiditis and Graves’ Disease
- Source :
- The Journal of Clinical Endocrinology & Metabolism. 90:5704-5710
- Publication Year :
- 2005
- Publisher :
- The Endocrine Society, 2005.
-
Abstract
- Context: Autoimmune thyroid diseases have been reported to be associated with human T cell leukemia virus type I (HTLV-I) infection. HTLV-I proviral load is related to the development of HTLV-I-associated myelopathy/tropical spastic paraparesis and has also been shown to be elevated in the peripheral blood of HTLV-I-infected patients with uveitis, arthritis, and connective tissue disease. Objective: The objective of the study was to evaluate the proviral load in HTLV-I-infected patients with Hashimoto’s thyroiditis (HT) or Graves’ disease (GD) and ascertain the ability of HTLV-I to infect thyroid cells. Patients and Methods: A quantitative real-time PCR assay was developed to measure the proviral load of HTLV-I in peripheral blood mononuclear cells from 26 HTLV-I-infected patients with HT, eight HTLV-I-infected patients with GD, or 38 asymptomatic HTLV-I carriers. Rat FRTL-5 thyroid cells were cocultured with HTLV-I-infected T cell line MT-2 or uninfected T cell line CCRF-CEM. After coculture with T cell lines, changes in Tax and cytokine mRNA expression were studied by RT-PCR. Results: HTLV-I proviral load was significantly higher in the peripheral blood of patients with HT and GD than asymptomatic HTLV-I carriers. In the peripheral blood from HTLV-I-infected patients with HT, HTLV-I proviral load did not correlate with the thyroid peroxidase antibody or thyroglobulin antibody titer. After coculture with MT-2 cells, FRTL-5 cells expressed HTLV-I-specific Tax mRNA. These cocultured FRTL-5 cells with MT-2 cells expressed IL-6 mRNA and proliferated more actively than those cocultured with CCRF-CEM cells. Conclusion: Our findings suggest the role of the retrovirus in the development of autoimmune thyroid diseases in HTLV-I-infected patients.
- Subjects :
- Male
viruses
Endocrinology, Diabetes and Metabolism
Graves' disease
Clinical Biochemistry
Biochemistry
Thyroiditis
Endocrinology
immune system diseases
hemic and lymphatic diseases
Tropical spastic paraparesis
Leukemia-Lymphoma, Adult T-Cell
Hashimoto Disease
RNA, Neoplasm
biology
Reverse Transcriptase Polymerase Chain Reaction
Thyroid
virus diseases
Middle Aged
Viral Load
Graves Disease
medicine.anatomical_structure
Cytokines
Female
Adult
endocrine system
medicine.medical_specialty
Leukemia, T-Cell
T cell
Peripheral blood mononuclear cell
Cell Line
Internal medicine
medicine
Animals
Humans
RNA, Messenger
Aged
Cell Proliferation
business.industry
Biochemistry (medical)
Thyroiditis, Autoimmune
medicine.disease
biology.organism_classification
HTLV-I Infections
Coculture Techniques
CD4 Lymphocyte Count
Rats
Human T-lymphotropic virus 1
DNA, Viral
Immunology
business
Subjects
Details
- ISSN :
- 19457197 and 0021972X
- Volume :
- 90
- Database :
- OpenAIRE
- Journal :
- The Journal of Clinical Endocrinology & Metabolism
- Accession number :
- edsair.doi.dedup.....6d1f66a5c62f3c858610e8bafea0958e
- Full Text :
- https://doi.org/10.1210/jc.2005-0679