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UV-aging of microplastics increases proximal ARG donor-recipient adsorption and leaching of chemicals that synergistically enhance antibiotic resistance propagation
- Source :
- Journal of hazardous materials. 427
- Publication Year :
- 2021
-
Abstract
- Despite growing attention to environmental pollution by microplastics (MP), the effects of MP aging on bacterial horizontal gene transfer (HGT) have not been systematically investigated. Here, we used UV-aged polystyrene microplastics (PS-MPs) to investigate how aging affects antibiotic resistance genes (ARGs) transfer efficiency from various ARG vectors to recipient bacteria. The adsorption capacity of MP20 (20-day UV-aged PS-MPs) towards E. coli (harboring plasmid-borne blaTEM-1), plasmid pET29 (harboring blaNDM-1) and phage lambda (carrying the aphA1 ARG) increased by 6.6-, 5.2- and 8.3-fold, respectively, relative to pristine PS-MPs (MP0), due to increased specific surface area and affinity for these ARG vectors. Moreover, MP20 released more organic compounds (TOC 1.6 mg/g-MP20, versus 0.2 mg/g-MP0 in 4 h) –possibly depolymerization byproducts (verified by GC-MS), which induced intracellular ROS generation, increased cell permeability and upregulated HGT associated genes. Accordingly, MP20 enhanced ARG transfer frequency from E. coli, plasmid pET29 and phage lambda (relative to MP0) by 1.3-, 4.7- and 3.5-fold, respectively. The Bliss independence model infers that higher bacterial adsorption and exposure to chemicals released during MP aging synergistically enhanced ARG transfer. This underscores the need to assess the significance of this overlooked phenomenon to the environmental dissemination of antibiotic resistance and other HGT processes.
- Subjects :
- Microplastics
Environmental Engineering
biology
Chemistry
Health, Toxicology and Mutagenesis
Environmental pollution
Drug Resistance, Microbial
Lambda phage
biology.organism_classification
Pollution
Molecular biology
Anti-Bacterial Agents
Plasmid
Horizontal gene transfer
Escherichia coli
Environmental Chemistry
Adsorption
Waste Management and Disposal
Gene
Plastics
Intracellular
Bacteria
Subjects
Details
- ISSN :
- 18733336
- Volume :
- 427
- Database :
- OpenAIRE
- Journal :
- Journal of hazardous materials
- Accession number :
- edsair.doi.dedup.....6d1b633b6c6233a79b0d0e85acd931fa