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Effectiveness of sampling methods employed for Acanthamoeba keratitis diagnosis by culture
- Source :
- International Ophthalmology. 39:1451-1458
- Publication Year :
- 2018
- Publisher :
- Springer Science and Business Media LLC, 2018.
-
Abstract
- This retrospective, observational study was designed to evaluate the effectiveness of the sampling methods commonly used for the collection of corneal scrapes for the diagnosis of Acanthamoeba keratitis (AK) by culture, in terms of their ability to provide a positive result. A total of 553 samples from 380 patients with suspected AK received at the Parasitology Section of the Public Health Institute of Chile, between January 2005 and December 2015, were evaluated. A logistic regression model was used to determine the correlation between the culture outcome (positive or negative) and the method for sample collection. The year of sample collection was also included in the analysis as a confounding variable. Three hundred and sixty-five samples (27%) from 122 patients (32.1%) were positive by culture. The distribution of sample types was as follows: 142 corneal scrapes collected using a modified bezel needle (a novel method developed by a team of Chilean corneologists), 176 corneal scrapes obtained using a scalpel, 50 corneal biopsies, 30 corneal swabs, and 155 non-biological materials including contact lens and its paraphernalia. Biopsy provided the highest likelihood ratio for a positive result by culture (1.89), followed by non-biological materials (1.10) and corneal scrapes obtained using a modified needle (1.00). The lowest likelihood ratio was estimated for corneal scrapes obtained using a scalpel (0.88) and cotton swabs (0.78). Apart from biopsy, optimum corneal samples for the improved diagnosis of AK can be obtained using a modified bezel needle instead of a scalpel, while cotton swabs are not recommended.
- Subjects :
- Male
medicine.medical_specialty
Acanthamoeba
Polymerase Chain Reaction
Cornea
03 medical and health sciences
0302 clinical medicine
Ophthalmology
Biopsy
Humans
Medicine
Eye Infections, Parasitic
Retrospective Studies
Microscopy, Confocal
medicine.diagnostic_test
biology
business.industry
DNA, Protozoan
biology.organism_classification
medicine.disease
Contact lens
Acanthamoeba Keratitis
Acanthamoeba keratitis
030221 ophthalmology & optometry
Female
Sample collection
business
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 15732630 and 01655701
- Volume :
- 39
- Database :
- OpenAIRE
- Journal :
- International Ophthalmology
- Accession number :
- edsair.doi.dedup.....6d19ae8e1b4d9604f014c01daa2f52fa