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Hyaluronan-positive plasma membrane protrusions exist on mesothelial cells in vivo
- Source :
- Histochemistry and cell biology. 145(5)
- Publication Year :
- 2016
-
Abstract
- Previous observations of our research group showed that HAS2 and HAS3 overexpression in cultured cells induces the formation of long and numerous microvillus-like cell protrusions, which are present also in cultured cell types with naturally high hyaluronan secretion and the cell protrusions resemble those found in mesothelial cells. The aim of this study was to investigate whether these hyaluronan secreting, actin-dependent protrusions exist also in vivo. It was found that rat mesothelium in vivo is positive for hyaluronan and Has1-3. Also microvilli in rat mesothelium and live primary cultures of mesothelial cells were found to be hyaluronan positive, and the cells expressed all Has isoforms. Furthermore, ultrastructure of the cell protrusions in rat mesothelium was similar to that induced by overexpression of HAS2 and HAS3, and the number and orientation of actin filaments supporting the cell protrusions was identical. The results of this study show that HA-positive protrusions exist in vivo and support the idea that hyaluronan secretion from plasma membrane protrusions is a general process. This mechanism is potentially crucial for the normal function and maintenance of tissues and body fluids and may be utilized in many therapeutic applications.
- Subjects :
- 0301 basic medicine
Male
Histology
Cell
Biology
Cell Membrane Structures
03 medical and health sciences
chemistry.chemical_compound
In vivo
Hyaluronic acid
medicine
Animals
Secretion
Hyaluronic Acid
Rats, Wistar
Molecular Biology
Actin
CD44
Epithelial Cells
Cell Biology
Cell biology
Rats
Mesothelium
Medical Laboratory Technology
030104 developmental biology
medicine.anatomical_structure
chemistry
biology.protein
Mesothelial Cell
Subjects
Details
- ISSN :
- 1432119X
- Volume :
- 145
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Histochemistry and cell biology
- Accession number :
- edsair.doi.dedup.....6d157b81480e68f18172d3aaf1073ff6