Back to Search
Start Over
Cellular Effects of Piezoelectric Versus Electrohydraulic High Energy Shock Waves
- Source :
- Journal of Urology. 147:486-490
- Publication Year :
- 1992
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 1992.
-
Abstract
- High energy shock waves produced by a piezoelectric lithotripter, (EDAP LT.01) and an electro-hydraulic lithotripter, (Dornier HM3) were examined for their effects on Chinese hamster ovary cells in suspension. The EDAP caused acute lactate dehydrogenase release, consistent with severe membrane disruption in a proportion of cells, with the remaining proportion of cells replicating normally as measured by clonogenic assay. Similarly, the Dornier also caused lactate dehydrogenase release. However, a significant proportion of cells which remained “viable” after Dornier treatment, (intact to lactate dehydrogenase), did not replicate by clonogenic assay. The Dornier HM3 lithotripter has been reported to produce free radicals in aqueous solution. In the current investigation, we could not detect significant free radical formation from the EDAP LT.01. Chinese hamster ovary cell killing by the Dornier HM3 was significantly augmented by radiosensitizers, 5-iodo-2-deoxyuridine or buthionine sulfoximine, while radioprotectors cysteamine and WR-1065 had no protective effect. EDAP cell killing was not influenced by either radioprotectors or radiosensitizers. The mechanism of in vitro cytotoxicity differs between piezoelectric and electrohydraulic high energy shock wave delivery.
- Subjects :
- medicine.medical_specialty
Cell Survival
Cysteamine
Urology
Radiation-Protective Agents
Cell Line
Colony-Forming Units Assay
chemistry.chemical_compound
Cricetulus
Cricetinae
Idoxuridine
Lithotripsy
Methionine Sulfoximine
Lactate dehydrogenase
Animals
Medicine
Buthionine sulfoximine
Clonogenic assay
Cytotoxicity
Buthionine Sulfoximine
L-Lactate Dehydrogenase
business.industry
Chinese hamster ovary cell
Ovary
Mercaptoethylamines
In vitro
Surgery
Cell killing
chemistry
Biophysics
Female
business
Cell Division
Subjects
Details
- ISSN :
- 15273792 and 00225347
- Volume :
- 147
- Database :
- OpenAIRE
- Journal :
- Journal of Urology
- Accession number :
- edsair.doi.dedup.....6d1539899f271060e65416641f952bd3