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Pioneer neutrophils release chromatin within in vivo swarms
- Source :
- eLife, Vol 10 (2021), eLife
- Publication Year :
- 2021
- Publisher :
- eLife Sciences Publications Ltd, 2021.
-
Abstract
- Neutrophils are rapidly recruited to inflammatory sites where their coordinated migration forms clusters, a process termed neutrophil swarming. The factors that modulate early stages of neutrophil swarming are not fully understood, requiring the development of new in vivo models. Using transgenic zebrafish larvae to study endogenous neutrophil migration in a tissue damage model, we demonstrate that neutrophil swarming is a conserved process in zebrafish immunity, sharing essential features with mammalian systems. We show that neutrophil swarms initially develop around an individual pioneer neutrophil. We observed the violent release of extracellular cytoplasmic and nuclear fragments by the pioneer and early swarming neutrophils. By combining in vitro and in vivo approaches to study essential components of neutrophil extracellular traps (NETs), we provide in-depth characterisation and high-resolution imaging of the composition and morphology of these release events. Using a photoconversion approach to track neutrophils within developing swarms, we identify that the fate of swarm-initiating pioneer neutrophils involves extracellular chromatin release and that the key NET components gasdermin, neutrophil elastase, and myeloperoxidase are required for the swarming process. Together our findings demonstrate that release of cellular components by pioneer neutrophils is an initial step in neutrophil swarming at sites of tissue injury.
- Subjects :
- 0301 basic medicine
Neutrophils
QH301-705.5
Science
Swarming (honey bee)
Extracellular Traps
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
0302 clinical medicine
Immunology and Inflammation
In vivo
Extracellular
Animals
Biology (General)
Zebrafish
Cell Aggregation
Peroxidase
General Immunology and Microbiology
biology
General Neuroscience
neutrophil
NETs
General Medicine
Neutrophil extracellular traps
biochemical phenomena, metabolism, and nutrition
biology.organism_classification
zebrafish
Chromatin
Cell biology
030104 developmental biology
neutrophil swarming
Neutrophil elastase
Myeloperoxidase
biology.protein
Wounds and Injuries
bacteria
Medicine
Leukocyte Elastase
030217 neurology & neurosurgery
Research Article
Subjects
Details
- Language :
- English
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- eLife
- Accession number :
- edsair.doi.dedup.....6d0a7ef42032b06a37e1b03dd6013727