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Pioneer neutrophils release chromatin within in vivo swarms

Authors :
Clare F. Muir
Catherine A. Loynes
Sultan Alasmari
Noémie Hamilton
Jack Hales
Fu Chuen Kon
Stephen A. Renshaw
Anastasia Kadochnikova
Katherine M. Henry
Visakan Kadirkamanathan
Maria-Cristina Keightley
Hannah M. Isles
Philip M. Elks
Graham J. Lieschke
Source :
eLife, Vol 10 (2021), eLife
Publication Year :
2021
Publisher :
eLife Sciences Publications Ltd, 2021.

Abstract

Neutrophils are rapidly recruited to inflammatory sites where their coordinated migration forms clusters, a process termed neutrophil swarming. The factors that modulate early stages of neutrophil swarming are not fully understood, requiring the development of new in vivo models. Using transgenic zebrafish larvae to study endogenous neutrophil migration in a tissue damage model, we demonstrate that neutrophil swarming is a conserved process in zebrafish immunity, sharing essential features with mammalian systems. We show that neutrophil swarms initially develop around an individual pioneer neutrophil. We observed the violent release of extracellular cytoplasmic and nuclear fragments by the pioneer and early swarming neutrophils. By combining in vitro and in vivo approaches to study essential components of neutrophil extracellular traps (NETs), we provide in-depth characterisation and high-resolution imaging of the composition and morphology of these release events. Using a photoconversion approach to track neutrophils within developing swarms, we identify that the fate of swarm-initiating pioneer neutrophils involves extracellular chromatin release and that the key NET components gasdermin, neutrophil elastase, and myeloperoxidase are required for the swarming process. Together our findings demonstrate that release of cellular components by pioneer neutrophils is an initial step in neutrophil swarming at sites of tissue injury.

Details

Language :
English
Volume :
10
Database :
OpenAIRE
Journal :
eLife
Accession number :
edsair.doi.dedup.....6d0a7ef42032b06a37e1b03dd6013727