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Aging Impairs Skeletal Muscle Mitochondrial Bioenergetic Function

Authors :
Rita Ferreira
Pedro Figueiredo
José Alberto Duarte
Hans-Joachim Appell
Scott K. Powers
Source :
The Journals of Gerontology Series A: Biological Sciences and Medical Sciences. :21-33
Publication Year :
2009
Publisher :
Oxford University Press (OUP), 2009.

Abstract

This study investigated the influence of age on the functional status of mitochondria isolated from skeletal muscle of C57BL/6 mice aged 3 and 18 months. We hypothesized that skeletal muscle mitochondria isolated from aged animals will exhibit a decreased respiratory function. Mitochondrial respiratory functional measures (ie, State 3 and 4 respiration, respiratory control ratio and number of nanomoles of ADP phosphorylated by nanomoles of O(2) consumed per mitochondrion) and biochemical markers of oxidative damage (aconitase activity, protein carbonyl derivatives, sulfhydryl groups, and malondialdehyde) were measured in isolated mitochondrial suspensions. Along with traditional tests of mitochondrial function, an in vitro repetitive ADP-stimulation test was used to evaluate the mitochondrial capacity to reestablish the homeostatic balance between successive ADP stimulations. The number of mitochondria per mitochondrial suspension, calculated by transmission electron microscopy, was used to normalize functional and biochemical data. Our results confirm the existence of an age-associated decline in mitochondrial function of mixed skeletal muscle, which is significantly correlated with higher levels of mitochondrial oxidative damage.

Details

ISSN :
1758535X and 10795006
Database :
OpenAIRE
Journal :
The Journals of Gerontology Series A: Biological Sciences and Medical Sciences
Accession number :
edsair.doi.dedup.....6d03242f148c9097fd3a93133999be12
Full Text :
https://doi.org/10.1093/gerona/gln048