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Aging Impairs Skeletal Muscle Mitochondrial Bioenergetic Function
- Source :
- The Journals of Gerontology Series A: Biological Sciences and Medical Sciences. :21-33
- Publication Year :
- 2009
- Publisher :
- Oxford University Press (OUP), 2009.
-
Abstract
- This study investigated the influence of age on the functional status of mitochondria isolated from skeletal muscle of C57BL/6 mice aged 3 and 18 months. We hypothesized that skeletal muscle mitochondria isolated from aged animals will exhibit a decreased respiratory function. Mitochondrial respiratory functional measures (ie, State 3 and 4 respiration, respiratory control ratio and number of nanomoles of ADP phosphorylated by nanomoles of O(2) consumed per mitochondrion) and biochemical markers of oxidative damage (aconitase activity, protein carbonyl derivatives, sulfhydryl groups, and malondialdehyde) were measured in isolated mitochondrial suspensions. Along with traditional tests of mitochondrial function, an in vitro repetitive ADP-stimulation test was used to evaluate the mitochondrial capacity to reestablish the homeostatic balance between successive ADP stimulations. The number of mitochondria per mitochondrial suspension, calculated by transmission electron microscopy, was used to normalize functional and biochemical data. Our results confirm the existence of an age-associated decline in mitochondrial function of mixed skeletal muscle, which is significantly correlated with higher levels of mitochondrial oxidative damage.
- Subjects :
- Male
Aging
medicine.medical_specialty
Bioenergetics
Muscle Proteins
Citrate (si)-Synthase
Mitochondrion
Biology
Mice
chemistry.chemical_compound
Microscopy, Electron, Transmission
Internal medicine
Respiration
medicine
Animals
Respiratory system
Muscle, Skeletal
Cytochromes c
Skeletal muscle
Articles
Malondialdehyde
Mitochondria, Muscle
Mice, Inbred C57BL
Oxidative Stress
Endocrinology
medicine.anatomical_structure
Biochemistry
chemistry
Phosphorylation
Electrophoresis, Polyacrylamide Gel
Lipid Peroxidation
Geriatrics and Gerontology
Energy Metabolism
Homeostasis
Subjects
Details
- ISSN :
- 1758535X and 10795006
- Database :
- OpenAIRE
- Journal :
- The Journals of Gerontology Series A: Biological Sciences and Medical Sciences
- Accession number :
- edsair.doi.dedup.....6d03242f148c9097fd3a93133999be12
- Full Text :
- https://doi.org/10.1093/gerona/gln048