Additional file 1 of A conserved female-specific larval requirement for MtnB function facilitates sex separation in multiple species of disease vector mosquitoes

Additional file 1: Fig. S1. Confirmation of shRNA expression in recombinant yeast strain MtnB.496. PCR reactions performed with primers corresponding to the MtnB.496 shRNA transcript generated a ~ 100 bp amplicon (see DNA standard at far left; cDNA template was prepared from MtnB.496 yeast total RNA). Negative control PCR reactions included an amplification with cDNA prepared from non-transformed yeast (marked by – sign) and a reaction with no cDNA added (marked by knot symbol). A representative ethidium bromide-stained agarose gel from one of two comparable biological replicate experiments is shown.