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Temporal and parental-specific expression of imprinted genes in a newly derived Chinese human embryonic stem cell line and embryoid bodies

Authors :
Zhihong Zhang
Yi Zhang
Weicheng Wang
Xiangyin Kong
Bo Wen Sun
Yu fei Zhu
Furong Gao
Gang Jin
Yun Feng
Ying Jin
Hua Jiang
Yi Juan Sun
Chunliang Li
A. Cong Yang
Shi Jun Fu
Source :
Human Molecular Genetics. 15:65-75
Publication Year :
2005
Publisher :
Oxford University Press (OUP), 2005.

Abstract

Although the study of imprinted genes in human development is very important, little is known about their expression and regulation in the early differentiation of human tissues due to lack of an appropriate model. In this study, a Chinese human embryonic stem (hES) cell line, SHhES1, was derived and fully characterized. Expression profiles of human imprinted genes were determined by Affymetrix Oligo micro-array in undifferentiated SHhES1 cells and SHhES1-derived embryoid bodies (EBs) at day 3, 8, 13 and 18. Thirty-two known human imprinted genes were detected in undifferentiated ES cells. Significantly, differential expression was found in nine genes at different stages of EB formation. Expression profile changes were confirmed by quantitative real-time reverse transcriptase-polymerase chain reaction in SHhES1 cells as well as in another independently derived hES cell line, HUES-7. In addition, the monoallelic expressions of four imprinted genes were examined in three different passages of undifferentiated ES cells and EBs of both hES cell lines. The monoallelic expressions of imprinted genes, H19, PEG10, NDNL1 and KCNQ1 were maintained in both undifferentiated hES cells and derived EBs. More importantly, with the availability of maternal peripheral blood lymphocyte sample, we demonstrated that the maternal expression of KCNQ1 and the paternal expression of NDNL1 and PEG10 were maintained in SHhES1 cells. These data provide the first demonstration that the parental-specific expression of imprinted genes is stable in EBs after extensive differentiation, also indicating that in vitro fertilization protocol does not disrupt the parental monoallelic expression of the imprinted genes examined.

Details

ISSN :
14602083 and 09646906
Volume :
15
Database :
OpenAIRE
Journal :
Human Molecular Genetics
Accession number :
edsair.doi.dedup.....6cf486d1f415fc7ac0f039ecc0783eb8