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M-CSF Accelerates Neointimal Formation in the Early Phase After Vascular Injury in Mice
- Source :
- Arteriosclerosis, Thrombosis, and Vascular Biology. 27:283-289
- Publication Year :
- 2007
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 2007.
-
Abstract
- Objective— Since the macrophage colony-stimulating factor (M-CSF) has been shown to stimulate differentiation and proliferation of monocyte/macrophage lineage and to be involved in the process of neointimal formation after vascular injury, we tested the effects of M-CSF on the recruitment of bone marrow-derived progenitor cells in neointimal formation after vascular injury in mice. Methods and Results— Wire-mediated vascular injury was produced in the femoral artery of C57BL/6 mice. Recombinant human M-CSF [500 μg/(kg·day)] or saline (control) was administered for 10 consecutive days, starting 4 days before the injury. Treatment with M-CSF accelerated neointimal formation in the early phase after injury, and this neointimal lesion mainly consisted of bone marrow-derived cells. M-CSF treatment had no effect on the mobilization of endothelial progenitor cells (EPCs: CD34 + /Flk-1 + ) and reendothelialization after injury. The stromal cell-derived factor-1 (SDF-1) was markedly expressed in the neointima and media after injury, whereas CXCR4 + cells were observed in the neointima. Further, a novel CXCR4 antagonist, AMD3100, significantly attenuated the M-CSF-induced neointimal formation. Conclusions— These findings suggest that M-CSF accelerated neointimal formation after vascular injury via the SDF-1–CXCR4 system, and the inhibition of this system has therapeutic potential for the treatment of cardiovascular diseases.
- Subjects :
- Male
Neointima
Benzylamines
Receptors, CXCR4
Pathology
medicine.medical_specialty
Stromal cell
Endothelium
medicine.medical_treatment
CD34
Bone Marrow Cells
Mice, Transgenic
Inflammation
Cyclams
Mice
Restenosis
Heterocyclic Compounds
medicine
Animals
Progenitor cell
Chemokine CCL2
Cell Proliferation
Interleukin-6
Tumor Necrosis Factor-alpha
business.industry
Macrophage Colony-Stimulating Factor
Stem Cells
Cell Differentiation
medicine.disease
Chemokine CXCL12
Interleukin-10
Mice, Inbred C57BL
Cytokine
medicine.anatomical_structure
Gene Expression Regulation
Endothelium, Vascular
medicine.symptom
Tunica Intima
Cardiology and Cardiovascular Medicine
business
Chemokines, CXC
Signal Transduction
Subjects
Details
- ISSN :
- 15244636 and 10795642
- Volume :
- 27
- Database :
- OpenAIRE
- Journal :
- Arteriosclerosis, Thrombosis, and Vascular Biology
- Accession number :
- edsair.doi.dedup.....6cf3213d61a56b75d8a9673bd80f1696