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High-Throughput Quantitation of Cannabinoids by Liquid Chromatography Triple-Quadrupole Mass Spectrometry
- Source :
- Molecules; Volume 27; Issue 3; Pages: 742, Molecules, Vol 27, Iss 742, p 742 (2022)
- Publication Year :
- 2021
-
Abstract
- The high-throughput quantitation of cannabinoids is important for the cannabis industry. As medicinal products increase, and research into compounds that have pharmacological benefits increase, and the need to quantitate more than just the main cannabinoids becomes more important. This study aims to provide a rapid, high-throughput method for cannabinoid quantitation using a liquid chromatography triple-quadrupole mass spectrometer (LC-QQQ-MS) with an ultraviolet diode array detector (UV-DAD) for 16 cannabinoids: CBDVA, CBDV, CBDA, CBGA, CBG, CBD, THCV, THCVA, CBN, CBNA, THC, Δ8-THC, CBL, CBC, THCA-A and CBCA. Linearity, limit of detection (LOD), limit of quantitation (LOQ), accuracy, precision, recovery and matrix effect were all evaluated. The validated method was used to determine the cannabinoid concentration of four different Cannabis sativa strains and a low THC strain, all of which have different cannabinoid profiles. All cannabinoids eluted within five minutes with a total analysis time of eight minutes, including column re-equilibration. This was twice as fast as published LC-QQQ-MS methods mentioned in the literature, whilst also covering a wide range of cannabinoid compounds.
- Subjects :
- quantitation
analysis
Cannabinoids
Plant Extracts
Pharmaceutical Science
Organic chemistry
Reproducibility of Results
high-throughput quantitation
Sensitivity and Specificity
Analytical Chemistry
High-Throughput Screening Assays
LC-QQQ-MS
QD241-441
Chemistry (miscellaneous)
Limit of Detection
Tandem Mass Spectrometry
cannabis
cannabinoids
Drug Discovery
Molecular Medicine
Physical and Theoretical Chemistry
Chromatography, High Pressure Liquid
Cannabis
Chromatography, Liquid
Subjects
Details
- ISSN :
- 14203049
- Volume :
- 27
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Molecules (Basel, Switzerland)
- Accession number :
- edsair.doi.dedup.....6cd3e04f15e1abfb3a06291c3d8b5bf1