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Wheat germ supplementation alleviates insulin resistance and cardiac mitochondrial dysfunction in an animal model of diet-induced obesity
- Source :
- British Journal of Nutrition. 118:241-249
- Publication Year :
- 2017
- Publisher :
- Cambridge University Press (CUP), 2017.
-
Abstract
- Obesity is strongly associated with insulin resistance (IR), along with mitochondrial dysfunction to metabolically active tissues and increased production of reactive O2 species (ROS). Foods rich in antioxidants such as wheat germ (WG), protect tissues from damage due to ROS and modulate some negative effects of obesity. This study examined the effects of WG supplementation on markers of IR, mitochondrial substrate metabolism and innate antioxidant markers in two metabolically active tissues (i.e. liver and heart) of C57BL/6 mice fed a high-fat–high-sucrose (HFS) diet. Male C57BL/6 mice, 6-week-old, were randomised into four dietary treatment groups (n 12 mice/group): control (C, 10 % fat kcal), C+10 % WG, HFS (60 % fat kcal) or HFS+10 % WG (HFS+WG). After 12 weeks of treatment, HFS+WG mice had significantly less visceral fat (−16 %, P=0·006) compared with the HFS group. WG significantly reduced serum insulin (P=0·009), the insulinotropic hormone, gastric inhibitory peptide (P=0·0003), and the surrogate measure of IR, homoeostatic model assessment of IR (P=0·006). HFS diet significantly elevated (45 %, P=0·02) cardiac complex 2 mitochondrial VO2, suggesting increased metabolic stress, whereas WG stabilised this effect to the level of control. Consequently, genes which mediate antioxidant defense and mitochondrial biogenesis (superoxide dismutase 2 (Sod2) and PPARγ coactivator 1-α (Pgc1a), respectively) were significantly reduced (PSod2 (P=0·048) but not Pgc1a. Together, these results showed that WG supplementation in HFS diet, reduced IR and improved cardiac mitochondrial metabolic functions.
- Subjects :
- Male
0301 basic medicine
medicine.medical_specialty
Antioxidant
medicine.medical_treatment
SOD2
Gene Expression
Medicine (miscellaneous)
030209 endocrinology & metabolism
Gastric Inhibitory Polypeptide
Intra-Abdominal Fat
Mitochondrion
Diet, High-Fat
Antioxidants
Superoxide dismutase
03 medical and health sciences
0302 clinical medicine
Insulin resistance
Internal medicine
medicine
Animals
Insulin
Obesity
Triticum
Nutrition and Dietetics
biology
Superoxide Dismutase
Chemistry
Myocardium
Heart
Metabolism
medicine.disease
Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
Mitochondria
Mice, Inbred C57BL
Disease Models, Animal
Oxidative Stress
030104 developmental biology
Endocrinology
Liver
Mitochondrial biogenesis
Dietary Supplements
biology.protein
Plant Preparations
Insulin Resistance
Reactive Oxygen Species
Hormone
Subjects
Details
- ISSN :
- 14752662 and 00071145
- Volume :
- 118
- Database :
- OpenAIRE
- Journal :
- British Journal of Nutrition
- Accession number :
- edsair.doi.dedup.....6ccf6a8ab22190f27db2a8c433068961