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Cerebrospinal Fluid Proteome Changes in Older Non-Cardiac Surgical Patients with Postoperative Cognitive Dysfunction
- Source :
- J Alzheimers Dis
- Publication Year :
- 2021
-
Abstract
- Background: Postoperative cognitive dysfunction (POCD), a syndrome of cognitive deficits occurring 1–12 months after surgery primarily in older patients, is associated with poor postoperative outcomes. POCD is hypothesized to result from neuroinflammation; however, the pathways involved remain unclear. Unbiased proteomic analyses have been used to identify neuroinflammatory pathways in multiple neurologic diseases and syndromes but have not yet been applied to POCD. Objective: To utilize unbiased mass spectrometry-based proteomics to identify potential neuroinflammatory pathways underlying POCD. Methods: Unbiased LC-MS/MS proteomics was performed on immunodepleted cerebrospinal fluid (CSF) samples obtained before, 24 hours after, and 6 weeks after major non-cardiac surgery in older adults who did (n = 8) or did not develop POCD (n = 6). Linear mixed models were used to select peptides and proteins with intensity differences for pathway analysis. Results: Mass spectrometry quantified 8,258 peptides from 1,222 proteins in > 50%of patient samples at all three time points. Twelve peptides from 11 proteins showed differences in expression over time between patients with versus without POCD (q
- Subjects :
- 0301 basic medicine
Male
Proteome
Disease
Proteomics
Bioinformatics
Article
Cohort Studies
03 medical and health sciences
0302 clinical medicine
Cerebrospinal fluid
Postoperative Cognitive Complications
Tandem Mass Spectrometry
Medicine
Humans
Neuroinflammation
Aged
business.industry
General Neuroscience
General Medicine
Perioperative
medicine.disease
Pathophysiology
Psychiatry and Mental health
Clinical Psychology
030104 developmental biology
Case-Control Studies
Female
Geriatrics and Gerontology
business
Postoperative cognitive dysfunction
030217 neurology & neurosurgery
Biomarkers
Subjects
Details
- ISSN :
- 18758908
- Volume :
- 80
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Journal of Alzheimer's disease : JAD
- Accession number :
- edsair.doi.dedup.....6cc5cdeb7d613609c9540eb0e3bcf79d