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MiR-107 inhibits the sprouting of intersegmental vessels of zebrafish embryos
- Source :
- Protoplasma. 259:691-702
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- MicroRNAs (miRNAs) play important roles in various biological processes. Our previous study showed that inhibition of MTOR with rapamycin treatment suppressed human endothelial cell tube formation, concomitant with the down-regulation of miR-107. Similarly, inhibition of Ztor by rapamycin also suppressed vascular development in zebrafish embryos. To gain a better understanding of the role of miR-107 and MTOR in vascular development, the present study sought to validate its function by over-expressing miR-107 in zebrafish embryos via microinjection with mimic miR-107 duplexes. Alkaline phosphatase (ALP) staining was used to visualise blood vessels in the zebrafish embryo, and expressions of Pten, Ztor and Rap1 were investigated by immunoblotting. Over-expression of miR-107 in zebrafish embryos inhibited the sprouting of intersegmental vessels (ISVs) with concomitant down-regulation of phosphorylated Rps6 expression, which confirmed the inhibition of Ztor signalling. As expected, pten, which is the target of miR-107, was down-regulated. Interestingly, Rap1, a small GTPase protein that is involved in intersomitic vessels sprouting during angiogenesis, was also down-regulated when miR-107 was over-expressed. Overall, our findings suggest that miR-107 and Ztor-mediated suppression of vascular development in zebrafish embryo involves Rap1.
- Subjects :
- Sirolimus
Tube formation
animal structures
biology
Angiogenesis
TOR Serine-Threonine Kinases
Down-Regulation
Cell Biology
Plant Science
General Medicine
biology.organism_classification
Cell biology
Endothelial stem cell
MicroRNAs
embryonic structures
biology.protein
Animals
PTEN
Rap1
Zebrafish
Microinjection
PI3K/AKT/mTOR pathway
Subjects
Details
- ISSN :
- 16156102 and 0033183X
- Volume :
- 259
- Database :
- OpenAIRE
- Journal :
- Protoplasma
- Accession number :
- edsair.doi.dedup.....6cc49f91168c3b9fca28eed470cfb8d7
- Full Text :
- https://doi.org/10.1007/s00709-021-01695-1