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Ex vivo generation of fully mature human red blood cells from hematopoietic stem cells
- Source :
- Nature Biotechnology. 23:69-74
- Publication Year :
- 2005
- Publisher :
- Springer Science and Business Media LLC, 2005.
-
Abstract
- We describe here the large-scale ex vivo production of mature human red blood cells (RBCs) from hematopoietic stem cells of diverse origins. By mimicking the marrow microenvironment through the application of cytokines and coculture on stromal cells, we coupled substantial amplification of CD34(+) stem cells (up to 1.95 x 10(6)-fold) with 100% terminal differentiation into fully mature, functional RBCs. These cells survived in nonobese diabetic/severe combined immunodeficient mice, as do native RBCs. Our system for producing 'cultured RBCs' lends itself to a fundamental analysis of erythropoiesis and provides a simple in vitro model for studying important human viral or parasitic infections that target erythroid cells. Further development of large-scale production of cultured RBCs will have implications for gene therapy, blood transfusion and tropical medicine.
- Subjects :
- Erythrocytes
Reticulocytes
Time Factors
Stromal cell
Ultraviolet Rays
Cellular differentiation
Cell Culture Techniques
Biomedical Engineering
CD34
Antigens, CD34
Bioengineering
Cell Separation
Mice, SCID
Biology
Applied Microbiology and Biotechnology
Hemoglobins
Mice
Mice, Inbred NOD
hemic and lymphatic diseases
medicine
Animals
Humans
Cells, Cultured
Erythroid Precursor Cells
Microscopy, Confocal
Stem Cells
Cell Differentiation
Genetic Therapy
Flow Cytometry
Hematopoietic Stem Cells
Coculture Techniques
Cell biology
Oxygen
Haematopoiesis
Red blood cell
medicine.anatomical_structure
Immunology
Cytokines
Molecular Medicine
Erythropoiesis
Stem cell
Ex vivo
Biotechnology
Subjects
Details
- ISSN :
- 15461696 and 10870156
- Volume :
- 23
- Database :
- OpenAIRE
- Journal :
- Nature Biotechnology
- Accession number :
- edsair.doi.dedup.....6caa21ff0931fc3318db1f4ef185d26e