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ERYTHROPOIETIN PROMOTES THE RECOVERY OF ERECTILE FUNCTION FOLLOWING CAVERNOUS NERVE INJURY
- Source :
- Journal of Urology. 174:2060-2064
- Publication Year :
- 2005
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 2005.
-
Abstract
- We investigated the effects of recombinant human (rh) erythropoietin (EPO) on erectile function recovery in a rat model of cavernous nerve (CN) injury.Male rats underwent unilateral CN transection and excision of a 5 mm segment of the contralateral CN. One group received rhEPO (5,000 U/kg) subcutaneously daily for 14 days, while another received rhEPO 1 day and 1 hour prior to nerve injury. An additional group of animals was pretreated with 1 dose of darbepoetin (25 microg/kg). At 14 days following CN injury rats underwent erection physiology studies. Axonal regeneration was evaluated by electron microscopy. EPO receptor expression in the penis and major pelvic ganglion was evaluated immunohistochemically and by real-time polymerase chain reaction.Daily rhEPO effectively recovered erections after CN injury compared with saline treatment. Maximal intracavernous pressure area under the curve normalized to mean arterial pressure was significantly greater in EPO treated vs saline treated animals (p0.05). rhEPO and darbepoetin pretreatment was as effective as continuous 14-day therapy. EPO receptor expression was localized to neuronal cell bodies of the major pelvic ganglion, penile nerves and endothelial cells in the penis. Electron microscopy revealed significant improvement in axonal regeneration in rhEPO treated animals 14 days following injury compared to controls.EPO receptors are expressed in local neuronal and vascular tissues. Exogenous administration of rhEPO or darbepoetin in the setting of CN injury promotes erectile function recovery. This occurs through axonal regeneration of the injured nerve and possible penile protection.
- Subjects :
- Male
medicine.medical_specialty
Mean arterial pressure
Urology
Receptor expression
medicine.medical_treatment
Molecular Sequence Data
Sensitivity and Specificity
Drug Administration Schedule
Rats, Sprague-Dawley
Random Allocation
Erectile Dysfunction
Internal medicine
Receptors, Erythropoietin
medicine
Animals
Erythropoietin
Saline
Probability
Analysis of Variance
Base Sequence
Dose-Response Relationship, Drug
Reverse Transcriptase Polymerase Chain Reaction
business.industry
Biopsy, Needle
Area under the curve
Nerve injury
medicine.disease
Immunohistochemistry
Recombinant Proteins
Nerve Regeneration
Rats
Disease Models, Animal
Microscopy, Electron
medicine.anatomical_structure
Endocrinology
Erectile dysfunction
medicine.symptom
business
Penis
medicine.drug
Subjects
Details
- ISSN :
- 15273792 and 00225347
- Volume :
- 174
- Database :
- OpenAIRE
- Journal :
- Journal of Urology
- Accession number :
- edsair.doi.dedup.....6c966b9cfc0485a0d1bc4dd892068623