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Casticin inhibits human prostate cancer DU 145 cell migration and invasion via Ras/Akt/NF-κB signaling pathways
- Source :
- Journal of food biochemistry. 43(7)
- Publication Year :
- 2018
-
Abstract
- Casticin, a polymethoxyflavone derived from natural plants, has biological activities including induction of cell apoptosis. In this study, we showed the beneficial effects of casticin on the inhibition of prostate cancer cell metastasis. Casticin reduced total viable cell number, thus, we selected low doses of casticin for following experiments. Casticin decreased cell mobility, suppressed cell migration and invasion, and reduced cell gelatinolytic activities of MMP-2/-9. Furthermore, casticin inhibited the protein levels of AKT, GSK3 αβ, Snail, and MMPs (MMP-2, -9, -13, and -7) at 24 and 48 hr treatment. Casticin diminished the expressions of NF-κB p65, GRB2, SOS-1, MEK, p-ERK1/2, and p-JNK1/2 at 48 hr treatment only. However, casticin reduced the level of E-cadherin at 24 hr treatment but elevated at 48 hr. The novel findings suggest that casticin may represent a new and promising therapeutic agent for the metastatic prostate cancer. PRACTICAL APPLICATIONS: Casticin derived from natural plants had been used for Chinese medicine in Chinese population for thousands of years. In the present study, casticin attenuated metastatic effects, including decreasing viable cell number, inhibiting the migration, invasion, and adhesion, and reducing matrix metalloproteinases activity on human prostate DU 145 cancer cells. In addition, the results also provided possible pathways involved in casticin anti-metastasis mechanism. We conclude that casticin may be an aptitude anticancer agent or adjuvant for the metastatic prostate cancer in the future.
- Subjects :
- Male
030309 nutrition & dietetics
Cell
Biophysics
Apoptosis
Metastasis
03 medical and health sciences
chemistry.chemical_compound
Prostate cancer
0404 agricultural biotechnology
Cell Movement
Cell Line, Tumor
medicine
Cell Adhesion
Humans
Neoplasm Invasiveness
Neoplasm Metastasis
Protein kinase B
Pharmacology
Flavonoids
0303 health sciences
Prostate
Transcription Factor RelA
Cancer
Prostatic Neoplasms
04 agricultural and veterinary sciences
Cell Biology
medicine.disease
040401 food science
Antineoplastic Agents, Phytogenic
Oncogene Protein v-akt
medicine.anatomical_structure
chemistry
Cancer cell
Casticin
Cancer research
ras Proteins
Food Science
Signal Transduction
Subjects
Details
- ISSN :
- 17454514
- Volume :
- 43
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Journal of food biochemistry
- Accession number :
- edsair.doi.dedup.....6c86734ff9f124fe93b8801ed3eb3911