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Expression and methylation data from SLE patient and healthy control blood samples subdivided with respect to ARID3a levels
- Source :
- Data in Brief, Data in Brief, Vol 9, Iss C, Pp 213-219 (2016)
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- Previously published studies revealed that variation in expression of the DNA-binding protein ARID3a in B lymphocytes from patients with systemic lupus erythematosus (SLE) correlated with levels of disease activity ("Disease activity in systemic lupus erythematosus correlates with expression of the transcription factor AT-rich-interactive domain 3A" (J.M. Ward, K. Rose, C. Montgomery, I. Adrianto, J.A. James, J.T. Merrill et al., 2014) [1]). The data presented here compare DNA methylation patterns from SLE peripheral blood mononuclear cells obtained from samples with high numbers of ARID3a expressing B cells (ARID3a(H)) versus SLE samples with normal numbers of ARID3a(+) B cells (ARID3a(N)). The methylation data is available at the gene expression omnibus (GEO) repository, "Gene Expression Omnibus: NCBI gene expression and hybridization array data repository" (R. Edgar, M. Domrachev, A.E. Lash, 2002) [2]. Isolated B cells from SLE ARID3a(H) and ARID3a(N) B samples were also evaluated via qRT-PCR for Type I interferon (IFN) signature and pathway gene expression levels by qRT-PCR. Similarly, healthy control B cells and B cells stimulated to express ARID3a with the TLR agonist, CpG, were also compared via qRT-PCR. Primers designed to detect 6 IFNa subtype mRNAs were tested in 4 IFNa, Epstein-Barr Virus-transformed B cell lines ("Reduced interferon-alpha production by Epstein-Barr virus transformed B-lymphoblastoid cell lines and lectin-stimulated lymphocytes in congenital dyserythropoietic anemia type I" (S.H. Wickramasinghe, R. Hasan, J. Smythe, 1997) [3]). The data in this article support the publication, "Human effector B lymphocytes express ARID3a and secrete interferon alpha" (J.M. Ward, M.L. Ratliff, M.G. Dozmorov, G. Wiley, J.M. Guthridge, P.M. Gaffney, J.A. James, C.F. Webb, 2016) [4].
- Subjects :
- 0301 basic medicine
SLE
Alpha interferon
ARID3a
Biology
lcsh:Computer applications to medicine. Medical informatics
Peripheral blood mononuclear cell
Congenital dyserythropoietic anemia type I
03 medical and health sciences
0302 clinical medicine
Interferon
Gene expression
medicine
lcsh:Science (General)
B cell
Data Article
B cells
Multidisciplinary
Methylation
medicine.disease
3. Good health
030104 developmental biology
medicine.anatomical_structure
030220 oncology & carcinogenesis
Immunology
DNA methylation
lcsh:R858-859.7
lcsh:Q1-390
medicine.drug
Subjects
Details
- ISSN :
- 23523409
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Data in Brief
- Accession number :
- edsair.doi.dedup.....6c783a6090e3451edb238877d7c033e3