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Intraurethral co-transplantation of bone marrow mesenchymal stem cells and muscle-derived cells improves the urethral closure
- Source :
- Stem Cell Research & Therapy, Vol 9, Iss 1, Pp 1-16 (2018), Stem Cell Research & Therapy
- Publication Year :
- 2018
-
Abstract
- Background Cell therapy constitutes an attractive alternative to treat stress urinary incontinence. Although promising results have been demonstrated in this field, the procedure requires further optimization. The most commonly proposed cell types for intraurethral injections are muscle derived cells (MDCs) and mesenchymal stem/stromal cell (MSCs). The aim of this study was to evaluate the effects of MDC-MSC co-transplantation into the urethra. Methods Autologous transplantation of labeled MDCs, bone marrow MSCs or co-transplantation of MDC-MSC were performed in aged multiparous female goats (n = 6 in each group). The mean number of cells injected per animal was 29.6 × 106(± 4.3 × 106). PBS-injected animals constituted the control group (n = 5). Each animal underwent urethral pressure profile (UPP) measurements before and after the injection procedure. The maximal urethral closure pressure (MUCP) and functional area (FA) of UPPs were calculated. The urethras were collected at the 28th or the 84th day after transplantation. The marker fluorochrome (DID) was visualized and quantified using in vivo imaging system in whole explants. Myogenic differentiation of the graft was immunohistochemically evaluated. Results The grafted cells were identified in all urethras collected at day 28 regardless of injected cell type. At this time point the strongest DID-derived signal (normalized to the number of injected cells) was noted in the co-transplanted group. There was a distinct decline in signal intensity between day 28 and day 84 in all types of transplantation. Both MSCs and MDCs contributed to striated muscle formation if transplanted directly to the external urethral sphincter. In the MSC group those events were rare. If cells were injected into the submucosal region they remained undifferentiated usually packed in clearly distinguishable depots. The mean increase in MUCP after transplantation in comparison to the pre-transplantation state in the MDC, MSC and MDC-MSC groups was 12.3% (± 11.2%, not significant (ns)), 8.2% (± 9.6%, ns) and 24.1% (± 3.1%, p = 0.02), respectively. The mean increase in FA after transplantation in the MDC, MSC and MDC-MSC groups amounted to 17.8% (± 15.4%, ns), 15.2% (± 12.9%, ns) and 17.8% (± 2.5%, p = 0.04), respectively. Conclusions The results suggest that MDC-MSC co-transplantation provides a greater chance of improvement in urethral closure than transplantation of each population alone.
- Subjects :
- Co-transplantation
0301 basic medicine
Urinary Incontinence, Stress
Urinary incontinence
030232 urology & nephrology
Medicine (miscellaneous)
Cell Count
0302 clinical medicine
Muscle-derived cells
Medicine
lcsh:QD415-436
lcsh:R5-920
education.field_of_study
Goats
Graft Survival
Cell Differentiation
3. Good health
medicine.anatomical_structure
Treatment Outcome
Molecular Medicine
Female
Stem cell
lcsh:Medicine (General)
Autologous transplantation
medicine.medical_specialty
Population
Urology
Bone Marrow Cells
Mesenchymal Stem Cell Transplantation
Biochemistry, Genetics and Molecular Biology (miscellaneous)
Transplantation, Autologous
lcsh:Biochemistry
03 medical and health sciences
Urethra
Animals
education
Muscle, Skeletal
Muscle Cells
business.industry
Urethral sphincter
Mesenchymal stem cell
Mesenchymal Stem Cells
Cell Biology
Transplantation
030104 developmental biology
Microscopy, Fluorescence
Bone marrow
Cell transplantation
business
Subjects
Details
- ISSN :
- 17576512
- Volume :
- 9
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Stem cell researchtherapy
- Accession number :
- edsair.doi.dedup.....6c6741f09c2d9a4881ed274bffc894b2