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CD82 supports survival of childhood acute myeloid leukemia cells via activation of Wnt/β-catenin signaling pathway
- Source :
- Pediatric Research. 85:1024-1031
- Publication Year :
- 2019
- Publisher :
- Springer Science and Business Media LLC, 2019.
-
Abstract
- Stem cell marker CD82 plays a vital role in the oncogenesis and progression of acute myelogenous leukemia (AML), especially in sharing properties of leukemia stem cells (LSCs). The Wnt/β-catenin pathway is required for the development of LSCs in AML. The present study aimed to validate whether CD82 supports the survival of LSCs in pediatric AML via activation of Wnt/β-catenin signaling pathway. CD82 expression and its correlation with molecules downstream of Wnt/β-catenin pathway in samples from pediatric AML patients were analyzed. Forced or downregulated expression of CD82 in AML cells was evaluated for the effects of CD82 on cell proliferation, cycle regulation, apoptosis, and adriamycin chemoresistance and to validate the underlying mechanism. Aberrant expression of CD82 in pediatric AML patients was found. CD82 messenger RNA expression correlated positively with downstream molecules of Wnt/β-catenin pathway in AML children. Knockdown of CD82 induced apoptosis, suppressed growth, and decreased adriamycin chemoresistance in AML cells. CD82 accelerated β-catenin nuclear location and then stimulated the expression of downstream molecules of Wnt/β-catenin pathway. CD82 regulates the proliferation and chemotherapy resistance of AML cells via activation of the Wnt/β-catenin pathway, which suggest that the CD82 may be a potential therapeutic target in AML children.
- Subjects :
- Cell Survival
Biology
Kangai-1 Protein
Stem cell marker
medicine.disease_cause
03 medical and health sciences
0302 clinical medicine
Cell Line, Tumor
hemic and lymphatic diseases
030225 pediatrics
medicine
Humans
Child
Wnt Signaling Pathway
neoplasms
beta Catenin
Gene knockdown
Cell growth
Wnt signaling pathway
medicine.disease
Leukemia, Myeloid, Acute
Leukemia
Pediatrics, Perinatology and Child Health
Cancer research
Stem cell
Signal transduction
Carcinogenesis
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 15300447 and 00313998
- Volume :
- 85
- Database :
- OpenAIRE
- Journal :
- Pediatric Research
- Accession number :
- edsair.doi.dedup.....6c639422cd418c3571fc76b583a12ea4