Back to Search
Start Over
Adenosine a2a receptors are upregulated in peripheral blood mononuclear cells from atrial fibrillation patients
- Source :
- Digital.CSIC. Repositorio Institucional del CSIC, instname, International Journal of Molecular Sciences, Vol 22, Iss 3467, p 3467 (2021), Dipòsit Digital de la UB, Universidad de Barcelona, International Journal of Molecular Sciences, INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau, Volume 22, Issue 7
- Publication Year :
- 2021
- Publisher :
- Molecular Diversity Preservation International, 2021.
-
Abstract
- Atrial fibrillation (AF) is the most common form of cardiac arrhythmia seen in clinical practice. While some clinical parameters may predict the transition from paroxysmal to persistent AF, the molecular mechanisms behind the AF perpetuation are poorly understood. Thus, oxidative stress, calcium overload and inflammation, among others, are believed to be involved in AF-induced atrial remodelling. Interestingly, adenosine and its receptors have also been related to AF development and perpetuation. Here, we investigated the expression of adenosine A2A receptor (A2AR) both in right atrium biopsies and peripheral blood mononuclear cells (PBMCs) from non-dilated sinus rhythm (ndSR), dilated sinus rhythm (dSR) and AF patients. In addition, plasma adenosine content and adenosine deaminase (ADA) activity in these subjects were also determined. Our results revealed increased A2AR expression in the right atrium from AF patients, as previously described. Interestingly, increased levels of adenosine content and reduced ADA activity in plasma from AF patients were detected. An increase was observed when A2AR expression was assessed in PBMCs from AF subjects. Importantly, a positive correlation (P=0.001) between A2AR expression in the right atrium and PBMCs was observed. Overall, these results highlight the importance of the A2AR in AF and suggest that the evaluation of this receptor in PBMCs may be potentially be useful in monitoring disease severity and the efficacy of pharmacological treatments in AF patients.<br />This work was supported by Ministerio de Ciencia, Innovación y Universidades–Agencia Estatal de Investigación-FEDER-UE (SAF2017-87349-R MICIU/AEI/FEDER/UE and SAF207-88019- C3-1R MICIU/AEI/FEDER/UE) and ISCIII/FEDER (PIE14/00034 and CIBERCV-RD12/0042/0002), Generalitat de Catalunya (2017SGR1604 and 2017SGR1769), Fundació la Marató de TV3 (Grant 20152030/31) and NIDDK Intramural Res. (ZIADK31117).
- Subjects :
- Male
Adenosine
Adenosina
Adenosine A2A receptor
030204 cardiovascular system & hematology
lcsh:Chemistry
0302 clinical medicine
Adenosine deaminase
Sinus rhythm
atrial fibrillation
Receptor
lcsh:QH301-705.5
Spectroscopy
Aged, 80 and over
Microscopy, Confocal
biology
Atrial fibrillation
General Medicine
Middle Aged
peripheral blood mononuclear cells
Up-Regulation
Computer Science Applications
adenosine
Female
Nuclis cel·lulars
medicine.symptom
medicine.drug
medicine.medical_specialty
Receptor, Adenosine A2A
Inflammation
Cell nuclei
Peripheral blood mononuclear cell
Article
Catalysis
Inorganic Chemistry
03 medical and health sciences
Internal medicine
Fibril·lació auricular
medicine
Humans
Heart Atria
Physical and Theoretical Chemistry
Molecular Biology
Aged
adenosine A2A receptor
business.industry
Organic Chemistry
Atrial Remodeling
medicine.disease
adenosine deaminase
HEK293 Cells
Endocrinology
lcsh:Biology (General)
lcsh:QD1-999
Peripheral blood mononuclear cells
Leukocytes, Mononuclear
biology.protein
business
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 14220067
- Database :
- OpenAIRE
- Journal :
- Digital.CSIC. Repositorio Institucional del CSIC, instname, International Journal of Molecular Sciences, Vol 22, Iss 3467, p 3467 (2021), Dipòsit Digital de la UB, Universidad de Barcelona, International Journal of Molecular Sciences, INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau, Volume 22, Issue 7
- Accession number :
- edsair.doi.dedup.....6c60d3a24621d0a1d77cb74e8a6ecdc8