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Homologous Recombination and Translesion DNA Synthesis Play Critical Roles on Tolerating DNA Damage Caused by Trace Levels of Hexavalent Chromium
- Source :
- PLoS ONE, PLoS ONE, Vol 11, Iss 12, p e0167503 (2016)
- Publication Year :
- 2016
-
Abstract
- Contamination of potentially carcinogenic hexavalent chromium (Cr(VI)) in the drinking water is a major public health concern worldwide. However, little information is available regarding the biological effects of a nanomoler amount of Cr(VI). Here, we investigated the genotoxic effects of Cr(VI) at nanomoler levels and their repair pathways. We found that DNA damage response analyzed based on differential toxicity of isogenic cells deficient in various DNA repair proteins is observed after a three-day incubation with K2CrO4 in REV1-deficient DT40 cells at 19.2 μg/L or higher as well as in TK6 cells deficient in polymerase delta subunit 3 (POLD3) at 9.8 μg/L or higher. The genotoxicity of Cr(VI) decreased ~3000 times when the incubation time was reduced from three days to ten minutes. TK mutation rate also significantly decreased from 6 day to 1 day exposure to Cr(VI). The DNA damage response analysis suggest that DNA repair pathways, including the homologous recombination and REV1- and POLD3-mediated error-prone translesion synthesis pathways, are critical for the cells to tolerate to DNA damage caused by trace amount of Cr(VI).
- Subjects :
- 0301 basic medicine
Chromium
Time Factors
lcsh:Medicine
medicine.disease_cause
Toxicology
Pathology and Laboratory Medicine
Biochemistry
Poultry
chemistry.chemical_compound
Natural Resources
Medicine and Health Sciences
Gamefowl
lcsh:Science
Homologous Recombination
Genetics
Multidisciplinary
Agriculture
6. Clean water
3. Good health
Nucleic acids
Vertebrates
Water Resources
Research Article
DNA Replication
Livestock
DNA repair
DNA damage
Cell Survival
DNA recombination
Biology
Cell Line
Birds
03 medical and health sciences
medicine
Animals
Humans
Hexavalent chromium
030102 biochemistry & molecular biology
DNA synthesis
Dose-Response Relationship, Drug
Biology and life sciences
Toxicity
lcsh:R
Ecology and Environmental Sciences
Organisms
DNA
Molecular biology
Carcinogens, Environmental
030104 developmental biology
chemistry
Fowl
Mutation
Amniotes
REV1
lcsh:Q
Chromium toxicity
Homologous recombination
Chickens
Genotoxicity
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 11
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- PloS one
- Accession number :
- edsair.doi.dedup.....6c5e1d4cb0d036fd99d4f79d6181a548