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Subacute oral administration of folic acid elicits anti-inflammatory response in a mouse model of allergic dermatitis
- Source :
- The Journal of Nutritional Biochemistry. 67:14-19
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- Folic acid (FA) deficiency is associated with several health problems, including megaloblastic anemia and fetal neural tube defects. Therefore, supplementation with FA is strongly recommended by governments worldwide. Recent published reports indicate that FA functions in immune system maintenance. The main objective of this study is to examine possible anti-inflammatory and antipruritic effects of FA using a mouse model of allergic dermatitis. The mouse model was developed by repetitive sensitization to the Th2-type hapten toluene-2,4-diisocyanate (TDI). During the development of allergic dermatitis, FA was orally administered to the mice at doses of 8, 160, 1000 or 10,000 μg/day for 5 weeks. The ear swelling response and scratching behavior were monitored after the TDI challenge. Serum, ear tissue and auricular lymph node samples were isolated for further analysis 24 h after the TDI challenge. The ear swelling response was reduced in a dose-dependent manner by FA administration, and a significant change was observed at a concentration of 10,000-μg/day group. Comparable results were obtained through histological evaluation and cytokine level measurement in the ear tissue samples. Oral administration of FA exhibited the inhibitory effect on T-cell infiltration and T-cell-related cytokine production in auricular lymph nodes. Scratching behavior was not altered by FA administration. The in vivo evidence was corroborated by in vitro results, which showed that FA treatment significantly interfered with T-cell proliferation in a dose-dependent manner. Our findings imply that subacute oral administration of FA elicits an anti-inflammatory response, mainly through inhibition of T-cell proliferation.
- Subjects :
- Keratinocytes
0301 basic medicine
Allergy
T-Lymphocytes
Endocrinology, Diabetes and Metabolism
medicine.medical_treatment
Clinical Biochemistry
Administration, Oral
Pharmacology
Biochemistry
03 medical and health sciences
Folic Acid
0302 clinical medicine
Oral administration
In vivo
medicine
Animals
Humans
030212 general & internal medicine
Megaloblastic anemia
Molecular Biology
Antipruritic
Sensitization
Cell Proliferation
Mice, Inbred BALB C
Nutrition and Dietetics
business.industry
Anti-Inflammatory Agents, Non-Steroidal
Antipruritics
medicine.disease
Mice, Inbred C57BL
Disease Models, Animal
Toxicity Tests, Subacute
030104 developmental biology
Cytokine
medicine.anatomical_structure
Dermatitis, Allergic Contact
Female
Lymph
business
medicine.drug
Subjects
Details
- ISSN :
- 09552863
- Volume :
- 67
- Database :
- OpenAIRE
- Journal :
- The Journal of Nutritional Biochemistry
- Accession number :
- edsair.doi.dedup.....6c57363112930ce236fc0e6c133a8b02