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Evidence for common nucleotide sequences in the RNA subunits comprising Rous sarcoma virus 70 S RNA
- Source :
- Virology. 61:287-291
- Publication Year :
- 1974
- Publisher :
- Elsevier BV, 1974.
-
Abstract
- Digestion of Rous sarcoma virus 32 P-labeled high molecular-weight RNA with T 1 ribonuclease resulted in the release of approximately 30 unique large oligonucleotides which were resolved by electrophoresis and chromatography on thin layers of cellulose and DEAE-cellulose. Determination of the radioactivity present in each oligonucleotide of known chain length and in the remaining digestion products made it possible to estimate the total number of residues expected in a polynucleotide chain containing the sequences represented. The oligonucleotides are present in amounts greater than would be expected for a unique and nonrepetitive 70 S RNA molecule with a molecular weight of 1 × 10 7 . Many of the sequences must, therefore, be present in more than one of the 35 S RNA subunits comprising the 70 S RNA.
- Subjects :
- Oligonucleotides
RNA-dependent RNA polymerase
Biology
Chromatography, DEAE-Cellulose
Ribonucleases
Virology
Nucleotide
Ribonuclease
chemistry.chemical_classification
Rous sarcoma virus
Base Sequence
Nucleotides
Oligonucleotide
RNA
biology.organism_classification
Molecular biology
Molecular Weight
Avian Sarcoma Viruses
chemistry
Polynucleotide
RNA editing
biology.protein
Autoradiography
RNA, Viral
Electrophoresis, Polyacrylamide Gel
Phosphorus Radioisotopes
Subjects
Details
- ISSN :
- 00426822
- Volume :
- 61
- Database :
- OpenAIRE
- Journal :
- Virology
- Accession number :
- edsair.doi.dedup.....6c570c5f8b3e7bc3a88ed6b16604f712