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Extracellular production of tellurium nanoparticles by the photosynthetic bacterium Rhodobacter capsulatus
- Source :
- Journal of hazardous materials, 309 (2016): 202–209. doi:10.1016/j.jhazmat.2016.02.011, info:cnr-pdr/source/autori:Borghese, Roberto; Brucale, Marco; Fortunato, Gianuario; Lanzi, Massimiliano; Mezzi, Alessio; Valle, Francesco; Cavallini, Massimiliano; Zannoni, Davide/titolo:Extracellular production of tellurium nanoparticles by the photosynthetic bacterium Rhodobacter capsulatus/doi:10.1016%2Fj.jhazmat.2016.02.011/rivista:Journal of hazardous materials (Print)/anno:2016/pagina_da:202/pagina_a:209/intervallo_pagine:202–209/volume:309
- Publication Year :
- 2016
-
Abstract
- The toxic oxyanion tellurite (TeO3(2-)) is acquired by cells of Rhodobacter capsulatus grown anaerobically in the light, via acetate permease ActP2 and then reduced to Te(0) in the cytoplasm as needle-like black precipitates. Interestingly, photosynthetic cultures of R. capsulatus can also generate Te(0) nanoprecipitates (TeNPs) outside the cells upon addition of the redox mediator lawsone (2-hydroxy-1,4-naphtoquinone). TeNPs generation kinetics were monitored to define the optimal conditions to produce TeNPs as a function of various carbon sources and lawsone concentration. We report that growing cultures over a 10 days period with daily additions of 1mM tellurite led to the accumulation in the growth medium of TeNPs with dimensions from 200 up to 600-700 nm in length as determined by atomic force microscopy (AFM). This result suggests that nucleation of TeNPs takes place over the entire cell growth period although the addition of new tellurium Te(0) to pre-formed TeNPs is the main strategy used by R. capsulatus to generate TeNPs outside the cells. Finally, X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FT-IR) analysis of TeNPs indicate they are coated with an organic material which keeps the particles in solution in aqueous solvents.
- Subjects :
- 0301 basic medicine
Environmental Engineering
Health, Toxicology and Mutagenesis
030106 microbiology
Malates
Infrared spectroscopy
chemistry.chemical_element
Oxyanion
Fructose
Photochemistry
Rhodobacter capsulatus
Lawsone
03 medical and health sciences
chemistry.chemical_compound
Tellurite
X-ray photoelectron spectroscopy
Pyruvic Acid
Organic chemistry
Environmental Chemistry
Lactic Acid
Photosynthesis
Waste Management and Disposal
Rhodobacter
Aqueous solution
biology
Rhodobacter capsulatu
Nanoprecipitates
Nanoprecipitate
biology.organism_classification
Pollution
Health, Toxicology and Mutagenesi
030104 developmental biology
chemistry
Photosynthetic bacteria
Nanoparticles
Tellurium
Naphthoquinones
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Journal of hazardous materials, 309 (2016): 202–209. doi:10.1016/j.jhazmat.2016.02.011, info:cnr-pdr/source/autori:Borghese, Roberto; Brucale, Marco; Fortunato, Gianuario; Lanzi, Massimiliano; Mezzi, Alessio; Valle, Francesco; Cavallini, Massimiliano; Zannoni, Davide/titolo:Extracellular production of tellurium nanoparticles by the photosynthetic bacterium Rhodobacter capsulatus/doi:10.1016%2Fj.jhazmat.2016.02.011/rivista:Journal of hazardous materials (Print)/anno:2016/pagina_da:202/pagina_a:209/intervallo_pagine:202–209/volume:309
- Accession number :
- edsair.doi.dedup.....6c4c0f39fbf97a9b897240bacd9b02c1