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Establishment and Characterization of a Fas-Resistant T Cell Line
- Source :
- Acta Haematologica. 102:22-30
- Publication Year :
- 1999
- Publisher :
- S. Karger AG, 1999.
-
Abstract
- Fas is a cell surface receptor that controls a signal transduction pathway leading to apoptosis. We established an antihuman Fas monoclonal antibody (mAb)-resistant variant, kit-225-FR, from the human T cell line, kit-225. Flow cytometric analysis revealed that the expression of Fas molecules on kit-225-FR was preserved. The defect in Fas molecule was not detected either by reverse transcription polymerase chain reaction (PCR) analysis of the Fas transcript or by PCR-single strand conformation polymorphism analysis of the Fas gene in kit-225-FR. Although kit-225-FR was resistant to a high concentration of anti-Fas mAb, apoptosis could be induced, as with the wild type, by exogenous C2-ceramide exposure. MORT1/FADD was expressed at wild-type level in kit-225-FR, as determined by Western blot analysis. It therefore appears that the apoptotic signal transduction in kit-225-FR is defective between FADD and the sphingomyelin-ceramide pathway. By comparing the differences from the wild-type kit-225, kit-225-FR would serve as a useful cell line for analyzing Fas-specific signal transduction pathways in detail.
- Subjects :
- biology
T-Lymphocytes
T cell
Apoptosis
Hematology
General Medicine
Protein-Tyrosine Kinases
Ceramides
Fas receptor
Molecular biology
Fas ligand
Reverse transcription polymerase chain reaction
medicine.anatomical_structure
Cell surface receptor
Tumor Cells, Cultured
biology.protein
medicine
Humans
fas Receptor
FADD
Phosphorylation
Signal transduction
Signal Transduction
Subjects
Details
- ISSN :
- 14219662 and 00015792
- Volume :
- 102
- Database :
- OpenAIRE
- Journal :
- Acta Haematologica
- Accession number :
- edsair.doi.dedup.....6c169c9d2953e5b66525e0b81b3d08d7