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CD138-negative clonogenic cells are plasma cells but not B cells in some multiple myeloma patients
- Source :
- Leukemia. 26(9)
- Publication Year :
- 2012
-
Abstract
- Clonogenic multiple myeloma (MM) cells reportedly lacked expression of plasma cell marker CD138. It was also shown that CD19(+) clonotypic B cells can serve as MM progenitor cells in some patients. However, it is unclear whether CD138-negative clonogenic MM plasma cells are identical to clonotypic CD19(+) B cells. We found that in vitro MM colony-forming cells were enriched in CD138(-)CD19(-)CD38(++) plasma cells, while CD19(+) B cells never formed MM colonies in 16 samples examined in this study. We next used the SCID-rab model, which enables engraftment of human MM in vivo. CD138(-)CD19(-)CD38(++) plasma cells engrafted in this model rapidly propagated MM in 3 out of 9 cases, while no engraftment of CD19(+) B cells was detected. In 4 out of 9 cases, CD138(+) plasma cells propagated MM, although more slowly than CD138(-) cells. Finally, we transplanted CD19(+) B cells from 13 MM patients into NOD/SCID IL2Rγc(-/-) mice, but MM did not develop. These results suggest that at least in some MM patients CD138-negative clonogenic cells are plasma cells rather than B cells, and that MM plasma cells including CD138(-) and CD138(+) cells have the potential to propagate MM clones in vivo in the absence of CD19(+) B cells.
- Subjects :
- Cancer Research
Plasma Cells
Mice, SCID
Immunophenotyping
Colony-Forming Units Assay
Interleukin 21
Mice
immune system diseases
Mice, Inbred NOD
hemic and lymphatic diseases
medicine
Animals
Humans
Clonogenic assay
Multiple myeloma
Bone Marrow Transplantation
B-Lymphocytes
CD40
biology
Hematology
medicine.disease
Molecular biology
Leukemia
Haematopoiesis
Oncology
biology.protein
Rabbits
Syndecan-1
Stem cell
Multiple Myeloma
Subjects
Details
- ISSN :
- 14765551
- Volume :
- 26
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- Leukemia
- Accession number :
- edsair.doi.dedup.....6c150f8775ecc1557c51cc45a20ba465