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Decreased expression of P2X7 in endometrial epithelial pre-cancerous and cancer cells
- Source :
- Gynecologic Oncology. 106:233-243
- Publication Year :
- 2007
- Publisher :
- Elsevier BV, 2007.
-
Abstract
- Objectives. To understand the potential role of P2X7 as biomarker of endometrial cancer, and the molecular mechanisms by which cancerous epithelial cells maintain low expression of P2X7. Methods. Feasibility clinical experimental study. Normal (28), simple or complex hyperplasia (7), complex hyperplasia with atypia (6) and cancer endometrial discarded tissues (40) were obtained from a total of 81 women, ages 25–75. Endpoint for P2X7 protein was average pixel signal density of tissue immunoreactivity with anti-P2X7 antibody. Endpoint for P2X7 mRNA was one-step quantitative Real-Time PCR. Experiments in-vitro included normal (hEVEC) and cancerous cervical epithelial cells (HeLa) transfected with reporter plasmid containing luciferase-3′ untranslated region (3′UTR)-P2X7 cDNA, using as endpoint steady-state luciferase mRNA levels. Results. Levels of P2X7 protein and mRNA were significantly lower in vivo, in tissues of complex hyperplasia with atypia or endometrial adenocarcinoma, than in tissues of normal endometrium, simple hyperplasia or complex hyperplasia tissues (sensitivity and specificity of 89– 100%, p
- Subjects :
- Adult
Pathology
medicine.medical_specialty
Blotting, Western
Endometrium
Article
HeLa
Biomarkers, Tumor
medicine
Humans
RNA, Messenger
Aged
Messenger RNA
biology
Receptors, Purinergic P2
Endometrial cancer
Obstetrics and Gynecology
Cancer
Epithelial Cells
Transfection
Middle Aged
Hyperplasia
medicine.disease
biology.organism_classification
Molecular biology
Endometrial Neoplasms
medicine.anatomical_structure
Oncology
Cancer cell
Female
Receptors, Purinergic P2X7
Precancerous Conditions
Subjects
Details
- ISSN :
- 00908258
- Volume :
- 106
- Database :
- OpenAIRE
- Journal :
- Gynecologic Oncology
- Accession number :
- edsair.doi.dedup.....6c0d114356e0dcae981e4763889a3b52