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Replicative cis-advantage of Polyomavirus regulatory region mutants in different murine cell lines

Authors :
Paolo Amati
V De Simone
DE SIMONE, Vincenzo
Amati, P.
Publication Year :
1987
Publisher :
American Society for Microbiology / DC:1752 N Street Northwest:Washington, DC 20036:(202)737-3600, EMAIL: jadelman@asmusa.org, INTERNET: http://www.asmusa.org/asm.htm, Fax: (202)942-9342, 1987.

Abstract

To determine the relative growth advantages of polyomavirus regulatory region mutants selected from Friend leukemic and neuroblastoma cells persistently infected with polyomavirus A2 wild type, different murine cell lines, some of which are capable of further differentiation in vitro, were used as hosts in mixed infection and transfection. The tests allowed the determination, through the measurement of cis-advantage in replication, of the most effective polyomavirus regulatory region constitutions for a given cell line and, in some cases, for specific stages of cell differentiation. Furthermore, different domains of the viral regulatory region were shown to have different effects--positive, neutral, or negative--depending on the host analyzed.

Details

Database :
OpenAIRE
Accession number :
edsair.doi.dedup.....6bff50febdb14c621494d01c1a4ce3fc