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Effects of Prostaglandins E2 and F2α on the in vitro maturation of bovine oocytes
- Source :
- Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice), Empresa Brasileira de Pesquisa Agropecuária (Embrapa), instacron:EMBRAPA
- Publication Year :
- 2019
-
Abstract
- We aimed to elucidate the effects of PGE2 and PGF2a on the in vitro maturation (IVM) of bovine oocytes. First, cumulus-oocyte complexes were matured in the media supplemented with or without PGE2, PGF2a, or PGE2 plus PGF2a for the final 24, 12, or 6 h of culture. Then, the cumulus-oocyte complexes were matured in the absence or presence of a PG endoperoxide synthase 2 (PTGS2) enzyme inhibitor (NS398) supplemented with PGE2, PGF2a, or PGE2 plus PGF2a. Finally, the expression of genes associated with PGs activity in cumulus cells (PTGS2, PG E-synthase-1 [PTGES1], and aldo-keto reductase 1 [AKR1B1]) or oocytes (receptors for PGE2 [PTGER2] and PGF2a [PTGFR]) of different competencies was quantified. Supplementation of the IVM medium with PGs did not improve in vitro embryo production or embryo quality (P > 0.05). During maturation, the relative abundance of PTGS2 transcripts increased (P < 0.05) only in the less-competent group, whereas those of PTGES1 increased in the less-competent and in the more-competent groups. Conversely, AKR1B1 expression decreased only in the less-competent group (P < 0.05). Receptors for the PGE2 and PGF2a genes were very low or undetectable in oocytes. In conclusion, PGE2 and PGF2a are not recommended for media supplementation during maturation because they have no effect on embryo development. Although genes related to PGs activity are differentially expressed in cumulus cells of cumulus-oocyte complexes of different competence during maturation, the expression of PGE2 and PGF2a receptor genes was either not detectable or was detected at low levels in oocytes. Made available in DSpace on 2020-12-10T09:06:38Z (GMT). No. of bitstreams: 1 cpafro-18481.pdf: 594117 bytes, checksum: 2a43765a8d98e902ce4f1c4059ca2485 (MD5) Previous issue date: 2020
- Subjects :
- Animal reproduction
Reductase
Maturação
Dinoprost
F2a
Embryo Culture Techniques
Cultura In Vitro
0302 clinical medicine
Endocrinology
E2
Food Animals
Maturation
Embriogénese
Gene expression
Receptor
Sulfonamides
030219 obstetrics & reproductive medicine
biology
Chemistry
Gene Expression Regulation, Developmental
Embryo
04 agricultural and veterinary sciences
Enzyme inhibitor
Embryogenesis
Bovinocultura
lipids (amino acids, peptides, and proteins)
Embryo quality
endocrine system
medicine.medical_specialty
Reprodução Animal
In vitro studies
Real-Time Polymerase Chain Reaction
Dinoprostone
Andrology
03 medical and health sciences
Prostaglandins
Internal medicine
Ovulo
medicine
Animals
RNA, Messenger
Oócito
Nitrobenzenes
urogenital system
0402 animal and dairy science
Prostaglandina
040201 dairy & animal science
In vitro maturation
In Vitro Oocyte Maturation Techniques
biology.protein
Oocytes
Animal Science and Zoology
Cattle
Subjects
Details
- ISSN :
- 18790054
- Volume :
- 72
- Database :
- OpenAIRE
- Journal :
- Domestic animal endocrinology
- Accession number :
- edsair.doi.dedup.....6bf7a0da7162c2fc986c5a966484bc89