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An autotransporter display platform for the development of multivalent recombinant bacterial vector vaccines
- Source :
- Jong, W S P, Daleke, M H, Vikstrom, D, ten Hagen-Jongman ten, C M, de Punder, K, van der Wel, N N, van de Sandt, C E, Rimmelzwaan, G F, Follman, F, Agger, E, Andersen, P, de Gier, J-W & Luirink, J 2014, ' An autotransporter display platform for the development of multivalent recombinant bacterial vector vaccines. ', Microbial Cell Factories, vol. 13, 162, pp. 1-15 . https://doi.org/10.1186/s12934-014-0162-8, Microbial Cell Factories, 13. BioMed Central Ltd., Microbial Cell Factories, 13:162, 1-15. BioMed Central, Microbial Cell Factories
- Publication Year :
- 2014
-
Abstract
- Background The Autotransporter pathway, ubiquitous in Gram-negative bacteria, allows the efficient secretion of large passenger proteins via a relatively simple mechanism. Capitalizing on its crystal structure, we have engineered the Escherichia coli autotransporter Hemoglobin protease (Hbp) into a versatile platform for secretion and surface display of multiple heterologous proteins in one carrier molecule. Results As proof-of-concept, we demonstrate efficient secretion and high-density display of the sizeable Mycobacterium tuberculosis antigens ESAT6, Ag85B and Rv2660c in E. coli simultaneously. Furthermore, we show stable multivalent display of these antigens in an attenuated Salmonella Typhimurium strain upon chromosomal integration. To emphasize the versatility of the Hbp platform, we also demonstrate efficient expression of multiple sizeable antigenic fragments from Chlamydia trachomatis and the influenza A virus at the Salmonella cell surface. Conclusions The successful efficient cell surface display of multiple antigens from various pathogenic organisms highlights the potential of Hbp as a universal platform for the development of multivalent recombinant bacterial vector vaccines. Electronic supplementary material The online version of this article (doi:10.1186/s12934-014-0162-8) contains supplementary material, which is available to authorized users.
- Subjects :
- Salmonella typhimurium
Antigen delivery
Heterologous
Chlamydia trachomatis
Bioengineering
medicine.disease_cause
Applied Microbiology and Biotechnology
law.invention
Microbiology
Bacterial genetics
Multivalent
03 medical and health sciences
SDG 3 - Good Health and Well-being
law
Endopeptidases
Escherichia coli
Influenza A virus
medicine
Secretion
Recombinant live vaccine
Antigens, Viral
Bacterial Secretion Systems
030304 developmental biology
Antigens, Bacterial
0303 health sciences
biology
030306 microbiology
Research
Mycobacterium tuberculosis
Surface display
biology.organism_classification
3. Good health
Bacterial vaccine
Influenza Vaccines
Autotransporter
Salmonella enterica
Bacterial Vaccines
Recombinant DNA
Biotechnology
Subjects
Details
- ISSN :
- 14752859
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- Microbial Cell Factories
- Accession number :
- edsair.doi.dedup.....6be9fcfb3e14e38f03f55a24d682f40a