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Guidelines for reporting single-cell RNA-seq experiments

Authors :
Anja Füllgrabe
Deanne Taylor
John C. Marioni
Nancy George
Irene Papatheodorou
Silvie Fexova
Matthew Green
Jim Kent
Norman Morrison
Nils Gehlenborg
Alvis Brazma
Sarah A. Teichmann
Parisa Nejad
Mallory A. Freeberg
Richard H. Scheuermann
Bruce J. Aronow
Laura Clarke
Nicole Vasilevsky
Clay Fischer
Laura Huerta
Source :
Nat Biotechnol
Publication Year :
2020
Publisher :
Springer Science and Business Media LLC, 2020.

Abstract

Single-cell RNA-Sequencing (scRNA-Seq) has undergone major technological advances in recent years, enabling the conception of various organism-level cell atlassing projects. With increasing numbers of datasets being deposited in public archives, there is a need to address the challenges of enabling the reproducibility of such data sets. Here, we describe guidelines for a minimum set of metadata to sufficiently describe scRNA-Seq experiments, ensuring reproducibility of data analyses.

Details

ISSN :
15461696 and 10870156
Volume :
38
Database :
OpenAIRE
Journal :
Nature Biotechnology
Accession number :
edsair.doi.dedup.....6be0d3c77c3b69fab24e38d8f1126759
Full Text :
https://doi.org/10.1038/s41587-020-00744-z