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Analysis of an estrogen binding macromolecule in human pancreas by radioimmunoassay
- Source :
- Journal of Steroid Biochemistry. 26:439-442
- Publication Year :
- 1987
- Publisher :
- Elsevier BV, 1987.
-
Abstract
- A radioimmunoassay for quantitation of the human pancreatic estrogen binding protein (hEBP) was developed using polyclonal rabbit hEBP antiserum and iodinated purified hEBP. Parallel dose-response curves were obtained when serial dilutions of human serum and of cytosols obtained from human pancreas, prostate and colon were analyzed simultaneously with serial dilutions of purified hEBP standard. Very high levels of hEBP (500–1000 mg/kg wet weight) were found in normal pancreas. High as well as medium levels were found in pancreatic carcinoma tissue and medium values (0.1–1 mg/kg wet weight) in prostate, colon and ovarian tissue. Other tissues and serum from healthy volunteers showed low values, usually below 0.1 mg/kg. When serial dilutions of rat pancreatic cytosol were analyzed in the hEBP assay, [ 125 I]hEBP was displaced by the rat preparation, but the dose-response curves were not parallel to the standard curves, indicating similarity but non-identity between the estrogen binding proteins in human and in rat pancreas.
- Subjects :
- medicine.medical_specialty
Serial dilution
Colon
medicine.drug_class
Radioimmunoassay
Biochemistry
Endocrinology
Internal medicine
medicine
Animals
Humans
Estrogen binding
Pancreas
Antiserum
biology
Binding protein
Rats
medicine.anatomical_structure
Receptors, Estrogen
Polyclonal antibodies
Estrogen
biology.protein
Carrier Proteins
Subjects
Details
- ISSN :
- 00224731
- Volume :
- 26
- Database :
- OpenAIRE
- Journal :
- Journal of Steroid Biochemistry
- Accession number :
- edsair.doi.dedup.....6bcecf91f7a06d36cbc39368e0966469