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Characterisation of the adiponectin receptors: differential cell-surface expression and temporal signalling profiles of AdipoR1 and AdipoR2 are regulated by the non-conserved N-terminal trunks
- Publication Year :
- 2015
- Publisher :
- Elsevier Ireland Ltd, 2015.
-
Abstract
- The adiponectin axis regulates cardiometabolic and inflammatory tone making it an attractive therapeutic focus. Rudimentary understanding of the adiponectin receptors, AdipoR1 and AdipoR2, constrains our ability to target these atypical seven trans-membrane proteins. Here, we aimed to further elaborate the molecular details governing cell-surface expression and signal transduction by transient expression of AdipoR1 or AdipoR2 in HEK293 cells. Following serum starvation, adiponectin reduced cell-surface expression of both receptors, consistent with internalisation, and promoted phosphorylation of downstream effectors. Temporal phosphorylation profiles differed with AdipoR1 and AdipoR2 transduced signals peaking at 15 min and 24 h. Analysis of receptor chimeras showed that the non-conserved N-terminal trunks (AdipoR1(1-70) and AdipoR2(1-81)) define the temporal signalling profiles and contain multiple regions that promote or inhibit cell-surface expression, respectively. These findings highlight the importance of the non-conserved N-terminal trunks and demonstrate that cell-surface expression of AdipoR1 and AdipoR2 is required for effective coupling to downstream effectors. © 2015 Elsevier Ireland Ltd.
- Subjects :
- Cell
Cell Culture Techniques
CHO Cells
Biology
Biochemistry
Cricetulus
Endocrinology
medicine
Animals
Humans
Phosphorylation
Receptor
Molecular Biology
Adiponectin
Effector
Cell Membrane
HEK 293 cells
C130 Cell Biology
Cell biology
HEK293 Cells
Signalling
medicine.anatomical_structure
Gene Expression Regulation
Mutation
Receptors, Adiponectin
Signal transduction
Signal Transduction
B120 Physiology
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....6bb7ea4e1de33e0045178e1b2d05ec38