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The effects of monensin on transport of membrane components in the frog retinal photoreceptor. I. Light microscopic autoradiography and biochemical analysis
- Source :
- The Journal of Neuroscience. 4:1086-1092
- Publication Year :
- 1984
- Publisher :
- Society for Neuroscience, 1984.
-
Abstract
- We have explored the use of the Na+-H+ ionophore monensin as a potential tool for the investigation of membrane assembly and transport in retinal photoreceptors. Autoradiographic analysis of frog retinas incubated with [3H]leucine in the presence of monensin revealed a lack of concentrated silver grains (“bands”) at the base of the rod outer segments, in contrast to controls. This is indicative of a pronounced monensin-induced decrease in disc membrane assembly. Biochemical analyses of whole retinas and isolated rod outer segment membranes showed that protein synthesis (including opsin synthesis) was not significantly inhibited under these conditions, whereas passage of membrane protein to the rod outer segment was blocked. Glycerolipid synthesis was not significantly affected by monensin. The results suggest that membrane proteins (e.g., opsin) destined for incorporation into the rod outer segment must pass through the Golgi apparatus and demonstrate the potential utility of monensin for inhibiting aspects of marcomolecule transport in photoreceptors.
- Subjects :
- Opsin
Time Factors
genetic structures
Ionophore
Biology
symbols.namesake
chemistry.chemical_compound
Leucine
Animals
Photoreceptor Cells
Tissue Distribution
Monensin
Eye Proteins
Furans
General Neuroscience
Rana pipiens
Membrane Proteins
Biological Transport
Articles
Golgi apparatus
Membrane transport
Membrane
chemistry
Biochemistry
Membrane protein
symbols
Biophysics
Autoradiography
GRENOUILLE
sense organs
Subjects
Details
- ISSN :
- 15292401 and 02706474
- Volume :
- 4
- Database :
- OpenAIRE
- Journal :
- The Journal of Neuroscience
- Accession number :
- edsair.doi.dedup.....6ba7ec8f479dec617ebb0adedd5fcc94
- Full Text :
- https://doi.org/10.1523/jneurosci.04-04-01086.1984