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Structural analysis of capsular polysaccharides expressed by Burkholderia mallei and Burkholderia pseudomallei
- Source :
- Carbohydrate Research. 349:90-94
- Publication Year :
- 2012
- Publisher :
- Elsevier BV, 2012.
-
Abstract
- Capsular polysaccharides (CPSs) were isolated from O-polysaccharide deficient strains of Burkholderia mallei and Burkholderia pseudomallei using a modified hot phenol/water extraction procedure. Glycosyl composition, methylation, MALDI-TOF MS analyses as well as (1)H NMR spectroscopy including COSY, TOCSY, NOESY, HMBC and HSQC experiments identified the presence of two distinct CPS antigens in the samples exhibiting the following structures: This study confirms the ability of B. mallei to express a 6-deoxy-heptan CPS and represents the first report of a mannan CPS being expressed by these bacterial pathogens.
- Subjects :
- chemistry.chemical_classification
Burkholderia pseudomallei
biology
Chemistry
Organic Chemistry
General Medicine
biology.organism_classification
Polysaccharide
Burkholderia mallei
Biochemistry
Analytical Chemistry
Microbiology
carbohydrates (lipids)
chemistry.chemical_compound
Polysaccharides
Carbohydrate Conformation
Glycosyl
Carbohydrate conformation
Nuclear Magnetic Resonance, Biomolecular
Two-dimensional nuclear magnetic resonance spectroscopy
Bacterial Capsules
Heteronuclear single quantum coherence spectroscopy
Mannan
Subjects
Details
- ISSN :
- 00086215
- Volume :
- 349
- Database :
- OpenAIRE
- Journal :
- Carbohydrate Research
- Accession number :
- edsair.doi.dedup.....6ba7193d5c23fe90ebabb7ad5fc9295b