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Recombinant Reg3α protein protects against experimental acute pancreatitis in mice
- Source :
- Molecular and Cellular Endocrinology. 422:150-159
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- Regenerating gene 3α (Reg3α) protein is a trophic factor that stimulates cell and tissue proliferation, neogenesis and also acts against apoptosis and necrosis. In order to explore the potential roles of recombinant Reg3α (rReg3α), we produced a mature rReg3α polypeptide for direct administration in l-arginine (L-Arg) induced acute pancreatitis (AP) in mice. Our results showed that rReg3α stimulated cell proliferation through Erk1/2 and p38 phosphorylation and also cyclin D1 upregulation mediated by Akt/ATF-2 signaling. Moreover, rReg3α administration significantly reduced the pancreatic damage caused by L-Arg injection, as shown in histological examination and serum amylase, lipase and C-reactive protein (CRP) assays. Not only acinar cell necrosis but also apoptosis found in the pancreas of AP mice were alleviated by rReg3α. Finally, upregulated Bcl-2 and Bcl-xL and suppressed poly (ADP-ribose) synthetase/polymerase (PARP) levels were detected as being relevant to the mechanism of rReg3α protection. We therefore conclude that rReg3α acts as a protective polypeptide against AP in mice by enhancing Bcl-2 and Bcl-xL expressions and suppressing PARP level.
- Subjects :
- 0301 basic medicine
Necrosis
p38 mitogen-activated protein kinases
Apoptosis
Pancreatitis-Associated Proteins
Acinar Cells
Arginine
Biochemistry
Neogenesis
Cell Line
Mice
03 medical and health sciences
0302 clinical medicine
Endocrinology
Cyclin D1
Downregulation and upregulation
Antigens, Neoplasm
Biomarkers, Tumor
medicine
Acinar cell
Animals
Humans
Lectins, C-Type
Molecular Biology
Protein kinase B
Cell Proliferation
biology
biology.organism_classification
Molecular biology
Recombinant Proteins
Disease Models, Animal
030104 developmental biology
Gene Expression Regulation
Pancreatitis
Proto-Oncogene Proteins c-bcl-2
030220 oncology & carcinogenesis
Female
medicine.symptom
Signal Transduction
Subjects
Details
- ISSN :
- 03037207
- Volume :
- 422
- Database :
- OpenAIRE
- Journal :
- Molecular and Cellular Endocrinology
- Accession number :
- edsair.doi.dedup.....6b963cdd3d9c736486372734fc1fa4ba