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A Unique Combination of Inflammatory Cytokines Enhances Apoptosis of Thyroid Follicular Cells and Transforms Nondestructive to Destructive Thyroiditis in Experimental Autoimmune Thyroiditis
- Source :
- The Journal of Immunology. 168:2470-2474
- Publication Year :
- 2002
- Publisher :
- The American Association of Immunologists, 2002.
-
Abstract
- Treatment of cultured primary human thyroid cells with IFN-γ and TNF-α uniquely allows the induction of Fas-mediated apoptosis. To investigate the role of this cytokine combination in vivo, CBA/J mice were immunized with thyroglobulin and then injected with IFN-γ and TNF-α. Compared with control animals, mice treated with IFN-γ and TNF-α showed significantly sustained lymphocytic infiltration in the thyroid, which was associated with the destruction of portions of the follicular architecture at wk 6 after initial immunization. Furthermore, the number of apoptotic thyroid follicular cells was increased only in the thyroids from mice treated with the IFN-γ and TNF-α. We also analyzed the function of the Fas pathway in vivo in cytokine-treated mice by using an agonist anti-Fas Ab injected directly into the thyroid. Minimal apoptosis of thyroid epithelial cells was observed unless the mice were pretreated with IFN-γ and TNF-α. These data demonstrate that this unique combination of inflammatory cytokines facilitates the apoptotic destruction of thyroid follicular cells in experimental autoimmune thyroiditis, in a manner similar to what is observed in Hashimoto’s thyroiditis in humans.
- Subjects :
- CD4-Positive T-Lymphocytes
endocrine system
medicine.medical_specialty
endocrine system diseases
medicine.medical_treatment
Immunology
Thyroid Gland
Apoptosis
CD8-Positive T-Lymphocytes
Thyroglobulin
Thyroiditis
Proinflammatory cytokine
Autoimmune thyroiditis
Interferon-gamma
Mice
Cell Movement
Internal medicine
medicine
Animals
Immunology and Allergy
Autoantibodies
Thyroid Epithelial Cells
Tumor Necrosis Factor-alpha
business.industry
Thyroid
Thyroiditis, Autoimmune
Drug Synergism
medicine.disease
Cytokine
medicine.anatomical_structure
Endocrinology
Mice, Inbred CBA
Female
business
Subjects
Details
- ISSN :
- 15506606 and 00221767
- Volume :
- 168
- Database :
- OpenAIRE
- Journal :
- The Journal of Immunology
- Accession number :
- edsair.doi.dedup.....6b8cac20bd01161d316b06212db9b6d6