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Comparison of the allergenicity and Ole e 1 content of 6 varieties of Olea europaea pollen collected during 5 consecutive years
- Source :
- Annals of Allergy, Asthma & Immunology. 98:464-470
- Publication Year :
- 2007
- Publisher :
- Elsevier BV, 2007.
-
Abstract
- Background Numerous varieties of Olea europaea have been described in Mediterranean countries. Objective To investigate the immunochemical characteristics of 6 varieties of Olea europaea collected during 5 consecutive years. Methods The varieties Carrasqueno, Manzanillo, Acebuche (wild olive), Hojiblanco, Picual, and Nevado were analyzed. Pollen samples from each variety were collected for 5 consecutive years from the same cultivars by trained personnel. The antigenic and allergenic profiles of these extracts were evaluated by means of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot using the serum of 29 O europaea -allergic individuals. Ole e 1 was measured using enzyme-linked immunosorbent assay and purified Ole e 1 and rabbit polyclonal antibodies. Allergenic potency was determined using enzyme-linked immunosorbent assay inhibition and is expressed in histamine equivalent prick units per gram of raw material. Results Hojiblanco and Acebuche had the lowest mean ± SD Ole e 1 content in the 5 years (0.045 ± 0.029 and 0.059 ± 0.031 μg/μg of freeze-dried material, respectively). The variety with the highest mean ± SD Ole e 1 content was Picual (0.19 ± 0.075 μg/μg). Hojiblanco had the lowest total biological potency throughout the study. A positive correlation was obtained between rainfall in the winter months and total allergenicity of the 6 varieties. Conclusions The different varieties of O europaea pollen demonstrated great differences in allergenic potency and Ole e 1 content. These differences were maintained throughout the study, suggesting that they are due to genetic differences intrinsic to the varieties, although certain climatic effects may also play a role.
- Subjects :
- Pulmonary and Respiratory Medicine
Veterinary medicine
Rain
Immunology
Enzyme-Linked Immunosorbent Assay
medicine.disease_cause
Olea
Pollen
Botany
medicine
Humans
Immunology and Allergy
Potency
Cultivar
Plant Proteins
Gram
Gel electrophoresis
biology
Temperature
Allergens
Antigens, Plant
biology.organism_classification
Oleaceae
Olea europaea Pollen
Subjects
Details
- ISSN :
- 10811206
- Volume :
- 98
- Database :
- OpenAIRE
- Journal :
- Annals of Allergy, Asthma & Immunology
- Accession number :
- edsair.doi.dedup.....6b829b13b8425007a534897b700aa42a